Abstract
The insulin mimic, peroxide of vanadate (pervanadate), stimulated 35S-methionine incorporation into Xenopus oocyte protein in a Mg(2+)-dependent manner. Reducing the extracellular Mg2+ concentration from 1.0 to 0.1 mM decreased the pervanadate-stimulated component of incorporation by 35%; with 0.01 mM Mg2+ or lower, the pervanadate-stimulated component was abolished. In addition, reducing extracellular Mg2+ to 0.01 mM inhibited about 50% of the insulin-stimulated component of methionine incorporation. Mg2+ depletion had no effects on incorporation in controls or when protein synthesis was stimulated by Zn2+ or bovine growth hormone. Thus, not all substances that stimulated protein synthesis showed a dependence on extracellular Mg2+. Reducing extracellular Ca2+ had no effects on methionine incorporation in control cells or in cells stimulated by pervanadate or insulin. When oocytes maintained in a paraffin oil medium were brought into contact with a 0.5 microliter droplet of buffer containing the Mg2+ indicator dye, mag-fura-2, and pervanadate, apparent droplet Mg2+ decreased rapidly, indicating net uptake by the cells. Insulin also caused a net uptake of Mg2+. In contrast, apparent extracellular Mg2+ was constant when cells wer...Continue Reading
References
Dec 1, 1991·The American Journal of Physiology·S IshijimaM Tatibana
May 1, 1991·Diabetes·D P Thorne, T D Lockwood
Jun 1, 1991·The American Journal of Physiology·R D Grubbs
Apr 1, 1991·Cell Regulation·P HainautE Van Obberghen
Nov 1, 1990·Diabetes·S M BrichardJ C Henquin
Apr 22, 1988·Science·D S Miller
Mar 15, 1989·Biochemical Pharmacology·R E White, H C Hartzell
Dec 1, 1989·The American Journal of Physiology·S IshijimaM Tatibana
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S B Horowitz, L J Tluczek
Oct 31, 1989·Biochemistry·I G FantusB I Posner
Mar 1, 1989·The American Journal of Physiology·B RajuR E London
Dec 1, 1986·Cell Differentiation·R BelléR Ozon
Dec 1, 1988·Diabetes/metabolism Reviews·S R Kimball, L S Jefferson
May 1, 1987·Canadian Journal of Physiology and Pharmacology·F J Alvarez-LeefmansS M Gamiño
Mar 22, 1985·Science·C E HeyligerJ H McNeill
Aug 31, 1987·Biochemical and Biophysical Research Communications·S KadotaB I Posner
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R Iyengar, L Birnbaumer
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D S MillerS B Horowitz
May 11, 1983·Nucleic Acids Research·J M Sperrazza, L L Spremulli
May 1, 1982·Experimental Cell Research·H Sanui, H Rubin
Jan 1, 1995·Journal of Cellular Physiology·D M BarnesD S Miller
Citations
Apr 8, 2003·Nutrition·Michael J HaasArshag D Mooradian
May 7, 2002·Journal of Toxicology and Environmental Health. Part a·David M BarnesDavid S Miller
May 9, 1998·The Journal of Biological Chemistry·E A FeshchenkoA Y Tsygankov
Mar 17, 2000·The Journal of Nutrition·M A ReisL A Velloso