Magnesium Fluoride Forms Unique Protein Corona for Efficient Delivery of Doxorubicin into Breast Cancer Cells

Toxics
Hamed Al-BusaidiEzharul Hoque Chowdhury

Abstract

The efficacy of chemotherapy is undermined by adverse side effects and chemoresistance of target tissues. Developing a drug delivery system can reduce off-target side effects and increase the efficacy of drugs by increasing their accumulation in target tissues. Inorganic salts have several advantages over other drug delivery vectors in that they are non-carcinogenic and less immunogenic than viral vectors and have a higher loading capacity and better controlled release than lipid and polymer vectors. MgF₂ crystals were fabricated by mixing 20 mM MgCl₂ and 10 mM NaF and incubating for 30 min at 37 °C. The crystals were characterized by absorbance, dynamic light scattering, microscopic observance, pH sensitivity test, SEM, EDX and FTIR. The binding efficacy to doxorubicin was assessed by measuring fluorescence intensity. pH-dependent doxorubicin release profile was used to assess the controlled release capability of the particle-drug complex. Cellular uptake was assessed by fluorescence microscopy. Cytotoxicity of the particles and the drug-particle complex were assessed using MTT assay to measure cell viability of MCF-7 cells. Particle size on average was estimated to be <200 nm. The crystals were cubic in shape. The particles w...Continue Reading

References

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Methods Mentioned

BETA
Fluorescence
Infrared Spectroscopy
X-Ray
Dynamic light scattering
light scattering

Software Mentioned

zetasizer
CellSens Dimension
Varian Resolution Pro
PEAKS
GraphPad
Varian FTIR

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