PMID: 8985376Jan 1, 1997Paper

Magnesium-induced conformational change of packaging RNA for procapsid recognition and binding during phage phi29 DNA encapsidation

Journal of Virology
C Chen, P Guo

Abstract

Bacteriophage phi29 is typical of double-stranded DNA viruses in that its genome is packaged into a preformed procapsid during maturation. An intriguing feature of phi29 assembly is that a virus-encoded RNA (pRNA) is required for the packaging of its genomic DNA. Psoralen cross-linking, primer extension, and T1 RNase partial digestion revealed that pRNA had at least two conformations; one was able to bind procapsids, and the other was not. In the presence of Mg2+, one stretch of pRNA, consisting of bases 31 to 35, was confirmed to be proximal to base 69, as revealed by its efficient cross-linking by psoralen. Two cross-linking sites in the helical region were identified. Mg2+ induced a conformational change of pRNA that exposes the portal protein binding site by promoting the refolding of two strands of the procapsid binding region, resulting in the formation of pRNA-procapsid complexes. The procapsid binding region in this binding-competent conformation could not be cross-linked with psoralen. When the two strands of the procapsid binding region were fastened by cross-linking, pRNA could neither bind procapsids nor package phi29 DNA. A pRNA conformational change was also discernible by comparison of migration rates in native E...Continue Reading

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Citations

Nov 22, 2002·The Journal of Biological Chemistry·Dan Shu, Peixuan Guo
May 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·W J MeijerM Salas
May 25, 2016·RNA Biology·Yumeng Hao, Jeffrey S Kieft
Feb 26, 2011·The Journal of Biological Chemistry·Xiaobo Gu, Susan J Schroeder
Sep 5, 2008·Biomedical Microdevices·Jeong-Mi MoonRashid Bashir
May 23, 2006·Nucleic Acids Research·Mark A RobinsonNicola J Stonehouse
Jan 1, 1997·Journal of Virology·M Trottier, P Guo
Apr 17, 2007·Journal of Molecular Biology·Rockney AtzShelley Grimes
Nov 2, 2021·The Journal of Physical Chemistry. B·Boyang ZhuoJingyuan Li
Dec 14, 2011·Virology·Lina JakutytėRimantas Daugelavičius

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