Magnesium influences the discrimination and release of ADP by human RAD51

DNA Repair
Kang-Sup ShimRichard Fishel

Abstract

hRAD51 lacks cooperative DNA-dependent ATPase activity and appears to function with 5-10-fold less Mg2+ compared to RecA. We have further explored the effect of Mg2+ on adenosine nucleotide binding, ATPase, and DNA strand exchange activities. hRAD51 was saturated with the poorly hydrolyzable analog of ATP, ATPgammaS, at approximately 0.08 mM Mg2+. In contrast, > 0.5 mM Mg2+ was required to saturate hRAD51 with ADP. We found ADP to be a significantly less effective competitive inhibitor of the hRAD51 ATPase at low Mg2+ concentrations (0.08 mM). Mg2+ did not appear to affect the ability of ATPgammaS to competitively inhibit the hRAD51 ATPase. Low Mg2+ (0.08-0.12 mM) enhanced the steady-state ATPase of hRAD51 while higher Mg2+ concentration (> 0.3 mM) was inhibitory. At low Mg2+, hRAD51 appeared capable of nearly complete hydrolysis of available ATP, suggesting a lack of ADP product inhibition. There was a strong correlation between the amount of Mg2+ required for stable ADP binding and the inhibition of hRad51 strand exchange activity. Simultaneous inclusion of exogenous ATP and chelation of Mg2+ with EDTA significantly enhanced ADP-->ATP exchange by hRAD51. These studies are consistent with the hypothesis that Mg2+ influences th...Continue Reading

References

Jan 1, 1979·Archives of Oto-rhino-laryngology·S K Juhn, W D Ward
Jan 1, 1991·Annual Review of Biophysics and Biophysical Chemistry·S C Kowalczykowski
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M M Cox, I R Lehman
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·S C Kowalczykowski, R A Krupp
May 1, 1994·Trends in Biochemical Sciences·M M Cox
Mar 8, 1996·The Journal of Biological Chemistry·Q ShanR B Inman
Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·R C GuptaC M Radding
Jan 1, 1997·Progress in Nucleic Acid Research and Molecular Biology·A I Roca, M M Cox
Jan 1, 1997·Annual Review of Biochemistry·S R Sprang
Aug 1, 1997·Mutation Research·P BaumannS C West
Feb 21, 1998·Biochemistry·J WeberA E Senior
Apr 16, 1998·The Journal of Biological Chemistry·E A Namsaraev, P Berg
Jun 19, 1998·Frontiers in Bioscience : a Journal and Virtual Library·P R BiancoS C Kowalczykowski
Jul 25, 1998·Genes & Development·R Fishel
Sep 22, 1999·Molecular and Cellular Biology·C MorrisonS Takeda
Jan 1, 2001·The Journal of Biological Chemistry·S SigurdssonP Sung
Jul 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·X YuE H Egelman
Feb 13, 2002·The Journal of Biological Chemistry·Gregory Tombline, Richard Fishel
Feb 13, 2002·The Journal of Biological Chemistry·Gregory TomblineRichard Fishel
Feb 13, 2002·The Journal of Biological Chemistry·Gregory TomblineRichard Fishel
Nov 28, 2002·Molecular Cell·Jachen A SolingerWolf-Dietrich Heyer
Feb 22, 2003·The Journal of Biological Chemistry·Shelley L LusettiMichael M Cox
Oct 7, 2003·Annual Review of Microbiology·Michael M Cox
May 5, 2004·The Journal of Biological Chemistry·Kang Sup ShimRichard Fishel
Jul 6, 2004·Nature Structural & Molecular Biology·Adam B ConwayPhoebe A Rice

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Citations

Feb 20, 2010·The Journal of Biological Chemistry·Joyce H G LebbinkTitia K Sixma
Jan 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Masayoshi HondaMaria Spies
Jun 20, 2014·Nature Communications·Dmitry V BugreevAlexander V Mazin
Dec 13, 2007·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Sep 12, 2014·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Charles EsnaultBenoît Chénais
Aug 16, 2014·Biochemical Pharmacology·Anaïs NormandAxelle Renodon-Cornière
Aug 21, 2007·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Karin SöderlundMarie Stenmark Askmalm

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