Magnesium lithospermate B and rosmarinic acid, two compounds present in Salvia miltiorrhiza, have potent antiviral activity against enterovirus 71 infections

European Journal of Pharmacology
Yi-Ching ChungTzyy-Rong Jinn

Abstract

The aim of this study was to identify the active ingredients responsible for the anti-EV71 activity produced by Salvia miltiorrhiza extracts. A pGS-EV71 IRES-based bicistronic reporter assay platform was used for rapid analysis of compounds that could specifically inhibit EV71 viral IRES-mediated translation. The analysis identified 2 caffeic acid derivatives, magnesium lithospermate B (MLB) and rosmarinic acid (RA), which suppressed EV71 IRES-mediated translation at concentrations of 30μg/ml. We also found that MLB and RA inhibited EV71 infection when they were added to RD cells during the viral absorption stage. MLB had a low IC50 value of 0.09mM and a high TI value of 10.52. In contrast, RA had an IC50 value of 0.50mM with a TI value of 2.97. MLB and RA (100µg/ml) also reduced EV71 viral particle production and significantly decreased VP1 protein production. We propose that these two derivatives inhibit EV71 viral entry into cells and viral IRES activity, thereby reducing viral particle production and viral RNA expression and blocking viral VP1 protein translation. This study provides useful information for the development of anti-EV71 assays and reagents by demonstrating a convenient EV71 IRES-based bicistronic assay platfo...Continue Reading

References

Jul 28, 1988·Nature·R J Jackson
Dec 8, 1998·The Journal of Pediatrics·L C LumM Lambert
Jan 11, 2005·Current Medicinal Chemistry·Ren-Wang JiangKwok-Pui Fung
Apr 9, 2005·Biotechnology and Bioengineering·Jin-Ching LeeJohn T A Hsu
Aug 9, 2005·Virology·Adrian Chong Nyi SimChit Laa Poh
Nov 18, 2005·Journal of Clinical Pharmacology·Limin ZhouMoses Sing Sum Chow
Feb 1, 2007·The American Journal of Chinese Medicine·Bin-Wen WuJim-Tong Horng
Apr 19, 2008·Trends in Microbiology·Encarnación Martínez-Salas
Oct 29, 2008·Acta Pharmacologica Sinica·Ying-ju ChenTzong-yuan Wu
Mar 30, 2010·Microbes and Infection·Kuo-Feng WengShin-Ru Shih
May 4, 2011·The Journal of Infectious Diseases·Hui-Chen HungJohn T-A Hsu
Sep 15, 2011·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Qin-Chang ZhuTao Peng
Nov 1, 2011·World Journal of Gastroenterology : WJG·Jing LuMing-Liang He
Jan 29, 2013·European Journal of Medicinal Chemistry·Ying-Ju LinFuu-Jen Tsai
Feb 14, 2014·Journal of Biomedical Science·Chee Wah TanYoke Fun Chan
Mar 4, 2014·Expert Review of Anti-infective Therapy·Shih-Min Wang, Ching-Chuan Liu
Apr 15, 2014·Current Opinion in Virology·Peng-Nien Huang, Shin-Ru Shih

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Citations

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Nov 8, 2017·Animal Health Research Reviews·Mahmoud AlagawanyKuldeep Dhama
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Jul 8, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Pedro Ferreira-SantosJosé A Teixeira
May 11, 2018·Critical Reviews in Food Science and Nutrition·Min ShiGuoyin Kai
Jul 17, 2019·Current Drug Discovery Technologies·Khojasteh MalekmohammadRobert D E Sewell
Sep 24, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zhong-Yan ZhouJing-Yi Tang
Mar 4, 2021·Journal of Agricultural and Food Chemistry·Fazlurrahman KhanYoung-Mog Kim
May 4, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Minglong GuanXiaoyun Fan
Jun 3, 2021·International Journal of Molecular Sciences·Guus Gijsbertus Hubert van den AkkerTim Johannes Maria Welting
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