Magnesium starvation of Aerobacter aerogenes. 3. Protein metabolism.

Journal of Bacteriology
S L Marchesi, D Kennell

Abstract

The metabolism of the ribosomal and soluble protein components of Aerobacter aerogenes was examined during its incubation in a Mg(++)-deficient medium. Bacteria were exposed to leucine-H(3) during the exponential growth period preceding Mg(++) starvation, and extracts were prepared after intervals of starvation and were centrifuged through gradients of sucrose to separate ribosomal from soluble proteins. Ribosomal proteins synthesized during the preceding exponential growth were slowly lost from the ribosomes; after 8 hr of starvation, few, if any, sedimented with ribosomes. Losses of total protein, together with the known rate of ribosome decay during Mg(++) starvation, suggested that these ribosomal proteins are ultimately degraded to acid-soluble products and account for all protein lost by the starving cells. These conclusions were supported by studies of Mg(++) starvation in a uracil-requiring strain of A. aerogenes: during uracil starvation a smaller fraction of the proteins synthesized were ribosomal, and the fraction of protein which subsequently decayed during Mg(++) starvation was correspondingly less. During recovery from Mg(++) starvation, proteins, lost from disintegrated ribosomes, were not detectably reutilized i...Continue Reading

References

Jan 1, 1967·Journal of Bacteriology·D Kennell, A Kotoulas
Sep 1, 1965·Journal of Molecular Biology·D Nakada, M J Marquisee
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Apr 18, 1966·Biochimica Et Biophysica Acta·D Schlessinger, F Ben-Hamida
Jan 1, 1967·Journal of Bacteriology·D Kennell, A Kotoulas
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Citations

Aug 15, 1969·Annals of the New York Academy of Sciences·W E Wacker
Jan 1, 1967·Journal of Bacteriology·D Kennell, A Kotoulas
Jan 1, 1967·Journal of Bacteriology·D Kennell, A Kotoulas
Jun 1, 1977·Cell Differentiation·S P Yadav, H K Das
Jan 1, 1978·The International Journal of Biochemistry·G A George, F W Heaton
Jun 1, 1976·Journal of Bacteriology·F C KungD A Glaser

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