Magnesium starvation of Aerobacter aerogenes. I. Changes in nucleic acid composition.

Journal of Bacteriology
D Kennell, A Kotoulas

Abstract

Aerobacter aerogenes incubated in a medium containing all factors necessary for exponential growth except Mg(++) continued to synthesize nucleic acids and proteins for more than 70 hr, provided the major carbon source was in excess at all times. After 24 hr of Mg(++) starvation, deoxyribonucleic acid content in the culture had increased 10-fold. In contrast, the viable-cell count increased only about threefold during the first few hours and then remained approximately constant for the subsequent 70 hr. After specified intervals of Mg(++) starvation, extracts of the bacteria, or ribonucleic acid (RNA) purified from them, was centrifuged through gradients of sucrose to separate transfer RNA from ribosomal components. After correcting for losses, we obtained the following results. (i) There was a progressive rise in the content of transfer RNA competent to accept amino acids and during starvation it remained completely stable. (ii) In contrast, the contents of normally sedimenting ribosomal RNA and ribosomal subunits (30 and 50S) remained approximately constant for more than 24 hr. This did not result from stability of ribosomes made prior to starvation together with an inhibition of synthesis of new particles. Rather, ribosomes w...Continue Reading

References

Jan 1, 1967·Journal of Bacteriology·D Kennell, A Kotoulas
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Citations

Apr 1, 1972·FEBS Letters·E Tam LennetteD Apirion
Feb 14, 1990·European Journal of Biochemistry·J MeadorD Kennell
Jul 1, 1975·Antimicrobial Agents and Chemotherapy·R T WoodK P Munnelly
Jan 1, 1967·Journal of Bacteriology·S L Marchesi, D Kennell
Jan 1, 1967·Journal of Bacteriology·D Kennell, A Kotoulas
Sep 26, 1967·Biochimica Et Biophysica Acta·P S Cohen, H L Ennis
Jun 1, 1976·Journal of Bacteriology·F C KungD A Glaser

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