Magnesium sulfate and nimesulide have synergistic effects on rescuing brain damage after transient focal ischemia.

Journal of Neurotrauma
Liang-Chao WangKuen-Jer Tsai

Abstract

Magnesium sulfate and nimesulide are commonly used drugs with reported neuroprotective effects. Their combination as stroke treatment has the potential benefits of decreasing individual drug dosage and fewer adverse effects. This study evaluated their synergistic effects and compared a low-dose combination with individual drug alone and placebo. Sprague-Dawley rats underwent 90 min of focal ischemia with intraluminal suture occlusion of the middle cerebral artery followed by reperfusion. The rats were randomly assigned to receive one of the following treatments: placebo, magnesium sulfate (MgSO₄; 45 mg/kg) intravenously immediately after the induction of middle cerebral artery occlusion, nimesulide (6 mg/kg) intraperitoneally before reperfusion, and combined therapy. Three days after the ischemia-reperfusion insult, therapeutic outcome was assessed by 2,3,5-triphenyltetrazolium chloride staining and a 28-point neurological severity scoring system. Cyclooxygenase-2, prostaglandin E₂, myeloperoxidase, and caspase-3 expression after treatment were evaluated using Western blot analyses and immunohistochemical staining, followed by immunoreactive cell analysis using tissue cytometry. Only the combination treatment group showed a sig...Continue Reading

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Citations

May 9, 2012·Reviews in Endocrine & Metabolic Disorders·Joseph Nuñez
May 3, 2014·Journal of Traditional Chinese Medicine = Chung I Tsa Chih Ying Wen Pan·Quanfang ChenYanbin Wu
Mar 12, 2013·Pharmacology, Biochemistry, and Behavior·G Andrew MickleyNathanael R Wiles
May 12, 2015·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Eunkuk ParkHun-Kuk Park
Sep 8, 2018·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Ligen ShiJun Chen
Apr 28, 2019·International Journal of Molecular Sciences·Hung-Wei LiaoKuen-Jer Tsai

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
protein assay
ELISA

Software Mentioned

TissueQuest
ImageJ
HistoQuest

Related Concepts

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Brain Ischemia

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