Magnetic-Based Microfluidic Device for On-Chip Isolation and Detection of Tumor-Derived Exosomes

Analytical Chemistry
Huiying XuBang-Ce Ye

Abstract

Exosomes are membrane-enclosed phospholipid extracellular vesicles, which can act as mediators of intercellular communication. Although the original features endow tumor-derived exosomes great potential as biomarkers, efficient isolation and detection methods remain challenging. Here, we presented a two-stage microfluidic platform (ExoPCD-chip), which integrates on-chip isolation and in situ electrochemical analysis of exosomes from serum. To promote exosomes capture efficiency, an improved staggered Y-shaped micropillars mixing pattern was designed to create anisotropic flow without any surface modification. By combining magnetic enrichment based on specific phosphatidylserine-Tim4 protein recognition with a new signal transduction strategy in a chip for the first time, the proposed platform enables highly sensitive detection for CD63 positive exosomes as low as 4.39 × 103 particles/mL with a linear range spanning 5 orders of magnitude, which is substantially better than the existing methods. The reduced volume of sample (30 μL) and simple affinity method also make it ideal for rapid downstream analysis of complex biofluids within 3.5 h. As a proof-of-concept, we performed exosomes analysis in human serum and liver cancer pati...Continue Reading

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Citations

Jul 13, 2019·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Chung-An ChenKevin C-W Wu
Aug 3, 2019·Electrophoresis·Sergio Ayala-MarJosé González-Valdez
Nov 7, 2019·Chemical Communications : Chem Comm·Fang HeBang-Ce Ye
Sep 12, 2019·Expert Review of Molecular Diagnostics·Tania FranceschiniMichelangelo Fiorentino
Feb 19, 2020·Expert Review of Molecular Diagnostics·Amy Makler, Waseem Asghar
Feb 8, 2020·Biotechnology Journal·Guiying LiFang Yang
Apr 23, 2020·The Analyst·Sinuo YuXiaoli Zhu
May 7, 2019·Nuclear Medicine and Molecular Imaging·Do Won Hwang
Aug 28, 2020·Lab on a Chip·Yang LiuLeidong Mao
Apr 9, 2020·Frontiers in Chemistry·Rebeca Miranda-CastroNoemí de-Los-Santos-Álvarez
Sep 16, 2020·Analytical Methods : Advancing Methods and Applications·Houman Kholafazad Kordasht, Mohammad Hasanzadeh
Feb 2, 2019·Analytical and Bioanalytical Chemistry·Nongnoot Wongkaew
Apr 30, 2020·Electrophoresis·Sumera KhizarAbdelhamid Elaissari
Oct 9, 2020·Chemistry, an Asian Journal·Zejun JiangJianping Li
Feb 23, 2020·Biochimica Et Biophysica Acta. General Subjects·Cristina ZivkoPaola Luciani
Jan 15, 2021·Diagnostics·Ragaa Abd-Ellatief, Maha Ragaa Abd-Ellatief
Feb 24, 2021·ACS Applied Materials & Interfaces·Huiying XuBang-Ce Ye
Mar 6, 2021·Chemical Reviews·Lingling WuWeihong Tan
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Apr 4, 2021·Polymers·Isacco GualandiBeatrice Fraboni
Apr 13, 2021·Biosensors & Bioelectronics·Mehdi MohammadiAmir Sanati-Nezhad

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