Magnetic bead-sensing-platform-based chemiluminescence resonance energy transfer and its immunoassay application

Analytical Chemistry
Guoxin QinFanggui Ye

Abstract

A competitive immunoassay based on chemiluminescence resonance energy transfer (CRET) on the magnetic beads (MBs) is developed for the detection of human immunoglobulin G (IgG). In this protocol, carboxyl-modified MBs were conjugated with horseradish peroxidase (HRP)-labeled goat antihuman IgG (HRP-anti-IgG) and incubated with a limited amount of fluorescein isothiocyanate (FITC)-labeled human IgG to immobilize the antibody-antigen immune complex on the surface of the MBs, which was further incubated with the target analyte (human IgG) for competitive immunoreaction and separated magnetically to remove the supernatant. The chemiluminescence (CL) buffer (containing luminol and H(2)O(2)) was then added, and the CRET from donor luminol to acceptor FITC in the immunocomplex on the surface of MBs occured immediately. The present protocol was evaluated for the competitive immunoassay of human IgG, and a linear relationship between CL intensity ratio (R = I(425)/I(525)) and human IgG concentration in the range of 0.2-4.0 nM was obtained with a correlation coefficient of 0.9965. The regression equation was expressed as R = 1.9871C + 2.4616, and a detection limit of 2.9 × 10(-11) M was obtained. The present method was successfully appli...Continue Reading

References

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Citations

Apr 15, 2016·Scientific Reports·Hui ChenRichard C Willson
Oct 5, 2012·The Analyst·Heejin ChoJi Hoon Lee
Apr 30, 2017·Analytical and Bioanalytical Chemistry·Pengfei ZhaoHuiying Zhao
Dec 20, 2012·Chemical Communications : Chem Comm·Quanbo WangHuangxian Ju
Oct 21, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Qiunan XuHuangxian Ju
Jun 18, 2021·Advanced Materials·Xinyi LiuWanwan Li
Oct 30, 2012·Analytical Chemistry·Chen-I WangHuan-Tsung Chang
Aug 3, 2018·Analytical Chemistry·Xiaowen YanHongquan Zhang

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