Magnetic macromolecular cross linked enzyme aggregates (CLEAs) of glucoamylase

Enzyme and Microbial Technology
Shamraja Nadar, Virendra K Rathod

Abstract

This work illustrates the preparation of magnetic macromolecular glucoamylase CLEAs using dialdehydic pectin, as a cross linker instead of traditional glutaraldehyde. The effect of precipitators type and amount, cross linker concentration, cross linking time and amount of amino functionalized magnetic nanoparticles (AFMNs) on glucoamylase activity was studied. Glucoamylase magnetic macromolecular CLEAs prepared by precipitation in presence of AFMNs by ammonium sulfate were subsequently cross linked by dialdehydic pectin. After cross-linked by pectin, 95.4% activity recovery was achieved in magnetic macromolecular CLEAs, whereas in case of glutaraldehyde cross linker, 85.3% activity recovery was achieved. Magnetic macromolecular CLEAs showed 2.91 and 1.27 folds higher thermal stability as compared to free and magnetic glutaraldehyde CLEAs. In kinetics study, magnetic macromolecular CLEAs retained same Km values, whereas magnetic glutaraldehyde CLEAs showed higher Km value than free enzyme. The porous structure of magnetic macromolecular CLEAs was not only enhanced mass transfer toward macromolecular substrates, but also showed compression resistance for 5 consecutive cycles which was checked in terms of effectiveness factor. At ...Continue Reading

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Citations

Jul 28, 2016·Food Chemistry·Uttam V SojitraVirendra K Rathod
Jun 28, 2016·International Journal of Biological Macromolecules·Shamraja S NadarVirendra K Rathod
Aug 23, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Natália G GraebinRafael C Rodrigues
Aug 24, 2017·World Journal of Microbiology & Biotechnology·Shamraja S Nadar, Virendra K Rathod
Feb 2, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiu XingXiao-Qi Yu
Jul 28, 2020·Critical Reviews in Food Science and Nutrition·Asghar Taheri-KafraniRajender S Varma
Jan 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·José Renato GuimarãesPaulo Waldir Tardioli
Mar 18, 2020·Bioresource Technology·Sadegh SadeghzadehSeyed Mehdi Borghei
Mar 18, 2017·International Journal of Biological Macromolecules·Shamraja S NadarVirendra K Rathod
Sep 3, 2018·International Journal of Biological Macromolecules·Shamraja S Nadar, Virendra K Rathod
Jul 22, 2019·International Journal of Biological Macromolecules·Shamraja S Nadar, Virendra K Rathod
Jun 22, 2021·International Journal of Biological Macromolecules·Hui GuoJunqing Qian
Sep 7, 2017·Chemical Reviews·Roger A Sheldon, John M Woodley
Jul 28, 2018·Journal of Agricultural and Food Chemistry·Hongjie BianShiru Jia
Jan 6, 2017·Journal of Agricultural and Food Chemistry·Jiandong CuiShiru Jia
Dec 17, 2021·ChemSusChem·Flóra NagyDiána Balogh-Weiser

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