Magnetic micro-device for manipulating PC12 cell migration and organization

Lab on a Chip
N AlonO Shefi

Abstract

Directing neuronal migration and growth has an important impact on potential post traumatic therapies. Magnetic manipulation is an advantageous method for remotely guiding cells. In the present study, we have generated highly localized magnetic fields with controllable magnetic flux densities to manipulate neuron-like cell migration and organization at the microscale level. We designed and fabricated a unique miniaturized magnetic device composed of an array of rectangular ferromagnetic bars made of permalloy (Ni80Fe20), sputter-deposited onto glass substrates. The asymmetric shape of the magnets enables one to design a magnetic landscape with high flux densities at the poles. Iron oxide nanoparticles were introduced into PC12 cells, making the cells magnetically sensitive. First, we manipulated the cells by applying an external magnetic field. The magnetic force was strong enough to direct PC12 cell migration in culture. Based on time lapse observations, we analysed the movement of the cells and estimated the amount of MNPs per cell. We plated the uploaded cells on the micro-patterned magnetic device. The cells migrated towards the high magnetic flux zones and aggregated at the edges of the patterned magnets, corroborating tha...Continue Reading

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Citations

May 18, 2016·Journal of Nanobiotechnology·Michal MarcusOrit Shefi
Aug 23, 2017·Scientific Reports·Xiaoqing LvHongda Chen
Sep 12, 2018·Nanomaterials·Michal MarcusOrit Shefi
Mar 20, 2020·Scientific Reports·Sharon CohenOrit Shefi
Aug 5, 2018·Annals of Biomedical Engineering·Katerina SpyridopoulouKaterina Chlichlia
Apr 24, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Richard HarrisonVirginie Sottile
Jun 11, 2019·Frontiers in Bioengineering and Biotechnology·Milad Fathi-AchacheloueiNihal Engin Vrana
May 21, 2020·Micromachines·Adar HacohenOrit Shefi
Sep 12, 2020·International Journal of Molecular Sciences·Maud BongaertsMathieu Coppey
Jul 2, 2019·Biomedical Optics Express·Inbar YarivDror Fixler
Aug 14, 2019·Frontiers in Bioengineering and Biotechnology·Jessica L FunnellRyan J Gilbert
Jan 19, 2018·ACS Synthetic Biology·Abdullah A Mosabbir, Kevin Truong
Nov 23, 2019·ACS Applied Materials & Interfaces·Elina Haimov-TalmoudOrit Shefi

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