Magnetic molecularly imprinted polymer prepared by microwave heating for confirmatory determination of chloramphenicol in chicken feed using high-performance liquid chromatography-tandem mass spectrometry

Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
Sumate Kunsa-NgiemSomyote Sutthivaiyakit

Abstract

A magnetic molecularly imprinted polymer (MMIP) for chloramphenicol was prepared using a surface-imprinted and microwave-heating-induced polymerization method. The surfaces of the magnetic particles were first double-bond functionalized with 3-(trimethoxysilyl)propyl methacrylate (γ-MPS), followed by the copolymerization of 4-vinyl pyridine (4-VP) and trimethylolpropane trimethacrylate (TRIM) in the presence of chloramphenicol as a template and 1,1-azobis(cyclohexane-carbonitrile) (ABCN) as an initiator in a mixture of dimethyl sulfoxide and water with microwave heating at 80°C. The magnetic polymer possesses supraparamagnetic properties and was used to concentrate and cleanup chicken feed extract, followed by chromatographic separation using a Lichrospher®100 RP C8 column and detection with two multi-reaction monitoring transitions at m/z 321→ 152 and m/z 321→ 257. The mean recoveries obtained at two spiking levels were in the range of 94.6-100% The relative intra- and inter-day standard deviations were in the range of 1.4-2.6% and 5.1-5.7%, respectively. The detection limit of the method was 0.12 µg kg-1. This confirmatory method was successfully applied to determine chloramphenicol in chicken feed samples.

References

Apr 7, 2007·Journal of Chromatography. a·Valérie Pichon
Oct 3, 2008·Journal of Agricultural and Food Chemistry·Dong ZhangBang-Ce Ye
Oct 18, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Suxia ZhangJianzhong Shen
Nov 18, 2011·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Tsuyoshi TakaZahra F Chaudhry Bet
Dec 23, 2011·Journal of the American Chemical Society·Claudio BaggianiGianfranco Giraudi

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