Magnetic molecularly imprinted polymer nanoparticles based electrochemical sensor for the measurement of Gram-negative bacterial quorum signaling molecules (N-acyl-homoserine-lactones)

Biosensors & Bioelectronics
Hui JiangXiulan Sun

Abstract

We have developed a novel and economical electrochemical sensor to measure Gram-negative bacterial quorum signaling molecules (AHLs) using magnetic nanoparticles and molecularly imprinted polymer (MIP) technology. Magnetic molecularly imprinted polymers (MMIPs) capable of selectively absorbing AHLs were successfully synthesized by surface polymerization. The particles were deposited onto a magnetic carbon paste electrode (MGCE) surface, and characterized by electrochemical measurements. Differential Pulse Voltammetry (DPV) was utilized to record the oxidative current signal that is characteristic of AHL. The detection limit of this assay was determined to be 8×10(-10)molL(-1) with a linear detection range of 2.5×10(-9)molL(-1) to 1.0×10(-7)molL(-1). This Fe3O4@SiO2-MIP-based electrochemical sensor is a valuable new tool that allows quantitative measurement of Gram-negative bacterial quorum signaling molecules. It has potential applications in the fields of clinical diagnosis or food analysis with real-time detection capability, high specificity, excellent reproducibility, and good stability.

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Citations

Jan 19, 2016·Analytical and Bioanalytical Chemistry·Natalia Lopez-BarbosaJohann F Osma
Oct 12, 2019·The Analyst·Olja Simoska, Keith J Stevenson
Oct 26, 2018·Sensors·Elisabetta PrimiceriGiuseppe Maruccio
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May 11, 2020·Biotechnology Journal·Francis McEachernGeraldine Merle
Oct 6, 2020·SLAS Technology·Veronica R CampbellDaniel T Kamei
Jan 19, 2021·Journal of Electroanalytical Chemistry·Hüseyin Oğuzhan KayaSeda Nur Topkaya
Feb 25, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Soumya RajpalPrashant Mishra
Apr 6, 2021·Advances in Colloid and Interface Science·Ashis TripathyFilipe Samuel Silva
Jun 25, 2021·Analytical and Bioanalytical Chemistry·J MarfàM I Pividori
Aug 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dounia ElfadilDario Compagnone
May 15, 2018·ACS Sensors·Mandana AmiriSabine Szunerits

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