Abstract
Breast cancer is a major cause of morbidity and mortality in Western women. Tumor neoangiogenesis, the formation of new blood vessels from pre-existing ones, may be used as a prognostic marker for cancer progression. Clinical practice uses dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) to detect cancers based on increased blood flow and capillary permeability. However, DCE-MRI requires repeated injections of contrast media. Therefore we explored the use of noninvasive time-of-flight (TOF) MR angiography for serial studies of mouse mammary glands to measure the number and size of arteries feeding mammary glands with and without cancer. Virgin female C3(1) SV40 TAg mice (n=9), aged 18-20 weeks, were imaged on a 9.4 Tesla small animal scanner. Multislice T2-weighted (T2W) images and TOF-MRI angiograms were acquired over inguinal mouse mammary glands. The data were analyzed to determine tumor burden in each mammary gland and the volume of arteries feeding each mammary gland. After in vivo MRI, inguinal mammary glands were excised and fixed in formalin for histology. TOF angiography detected arteries with a diameter as small as 0.1 mm feeding the mammary glands. A significant correlation (r=0.79; p< 0.0001) was found...Continue Reading
References
Jun 5, 2003·Nature Medicine·Donald M McDonald, Peter L Choyke
Apr 6, 2004·Journal of Magnetic Resonance Imaging : JMRI·Shawn WagnerWolfgang Schaper
Sep 17, 2005·Cancer Research·Lauren M BrubakerWeili Lin
Sep 24, 2005·Academic Radiology·Elizabeth BullittMatthew G Ewend
Dec 12, 2007·The British Journal of Radiology·A RadjenovicM A Smith
Jul 26, 2008·NMR in Biomedicine·Lindsay W Turnbull
Nov 10, 2011·Technology in Cancer Research & Treatment·M HeijblomS Manohar
May 25, 2012·The British Journal of Radiology·J P B O'ConnorA Jackson
Feb 1, 2013·BMC Medical Informatics and Decision Making·Daiki KobayashiTsuguya Fukui
Feb 15, 2013·Thrombosis and Haemostasis·Josef EhlingFabian Kiessling
Jun 20, 2013·Cancer Prevention Research·Paul A VoldenSuzanne D Conzen
Nov 23, 2013·The American Journal of Pathology·Josef EhlingTwan Lammers
Jan 1, 2013·Magnetic Resonance Insights·Rebecca M MayrhoferPhilip W Kuchel
Dec 17, 2014·Breast Cancer Research : BCR·Xiaobing FanGregory S Karczmar
Feb 3, 2015·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Lin LiBaozhong Shen
Feb 25, 2015·Biointerphases·Lara J GamblePeggy Porter
May 16, 2015·Pathology, Research and Practice·Ji-Dong YanYue-Ming Hu
Jul 15, 2015·NMR in Biomedicine·Devkumar MustafiGregory S Karczmar
Apr 22, 2016·Magma·Maddalena StrumiaMichael Bock
May 3, 2016·Journal of the American Veterinary Medical Association·Chieko IshikawaToshihiro Watari
May 20, 2016·Abdominal Radiology·Rose M BermanBaris Turkbey
Aug 22, 2017·The American Journal of Pathology·Erin M McAuleyDonald J Vander Griend