Magnetic resonance imaging with superparamagnetic iron oxide fails to track the long-term fate of mesenchymal stem cells transplanted into heart

Scientific Reports
Ning MaShihua Zhao

Abstract

MRI for in vivo stem cell tracking remains controversial. Here we tested the hypothesis that MRI can track the long-term fate of the superparamagnetic iron oxide (SPIO) nanoparticles labelled mesenchymal stem cells (MSCs) following intramyocardially injection in AMI rats. MSCs (1 × 10(6)) from male rats doubly labeled with SPIO and DAPI were injected 2 weeks after myocardial infarction. The control group received cell-free media injection. In vivo serial MRI was performed at 24 hours before cell delivery (baseline), 3 days, 1, 2, and 4 weeks after cell delivery, respectively. Serial follow-up MRI demonstrated large persistent intramyocardial signal-voids representing SPIO during the follow-up of 4 weeks, and MSCs did not moderate the left ventricular dysfunction. The TUNEL analysis confirmed that MSCs engrafted underwent apoptosis. The histopathological studies revealed that the site of cell injection was infiltrated by inflammatory cells progressively and the iron-positive cells were macrophages identified by CD68 staining, but very few or no DAPI-positive stem cells at 4 weeks after cells transplantation. The presence of engrafted cells was confirmed by real-time PCR, which showed that the amount of Y-chromosome-specific SRY ...Continue Reading

References

May 7, 2003·Circulation·Dara L KraitchmanJeff W M Bulte
Apr 4, 2006·The Journal of Thoracic and Cardiovascular Surgery·Jiang-Yong MinJames P Morgan
Apr 4, 2006·Nature Medicine·Yoshinori MiyaharaHidezo Mori
Feb 22, 2008·Nature·Vincent F M Segers, Richard T Lee
Jun 10, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Carolyn A CarrKieran Clarke
Aug 8, 2008·Journal of Cellular and Molecular Medicine·Catharina NesselmannGustav Steinhoff
Nov 27, 2008·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Ian Y ChenSanjiv S Gambhir
Feb 27, 2010·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·E M WinterL van der Weerd
Mar 10, 2010·The American Journal of Cardiology·Jochen WöhrleVinzenz Hombach
Jun 26, 2010·European Journal of Heart Failure·Bodo-Eckehard StrauerChristiana M Schannwell
Nov 16, 2011·JAMA : the Journal of the American Medical Association·Jay H TraverseUNKNOWN Cardiovascular Cell Therapy ResearchNetwork
Mar 14, 2013·Journal of Cellular and Molecular Medicine·Claudio MuscariGianandrea Pasquinelli

❮ Previous
Next ❯

Citations

Feb 4, 2016·Stem Cells International·Kai ZhuChunsheng Wang
Dec 23, 2015·Annals of Biomedical Engineering·Daniela Y SantiestebanStanislav Emelianov
Aug 16, 2018·Scientific Reports·Andrei VenterHai-Ling Margaret Cheng
Aug 6, 2020·International Journal of Molecular Sciences·Na Kyung LeeDuk L Na
Jul 19, 2019·Contrast Media & Molecular Imaging·Inga E HaedickeHai-Ling Margaret Cheng
Feb 23, 2020·The Quarterly Journal of Nuclear Medicine and Molecular Imaging : Official Publication of the Italian Association of Nuclear Medicine (AIMN) [and] the International Association of Radiopharmacology (IAR), [and] Section of the Society of Radiopharmaceutica·Janette IkingChristoph Rischpler
Jan 14, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Liqin XiongFengwen Cao
Feb 9, 2019·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Hossein NejadnikHeike Daldrup-Link
Oct 16, 2020·Journal of Stroke·Shuai ZhangXiaofeng Jia
Feb 17, 2021·ACS Biomaterials Science & Engineering·Luis Eduardo Portillo Esquivel, Boyang Zhang
Jul 25, 2020·IScience·Daniel A SzulcHai-Ling Margaret Cheng
Sep 9, 2021·Emerging Topics in Life Sciences·Francesco AmadeoPatricia Murray

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
fluorescence microscopy

Software Mentioned

ImageJ

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis