Magnetic resonance spectroscopy for the determination of renal metabolic rate in vivo

Kidney International
D FreemanB Ross

Abstract

The method of magnetic resonance spectroscopy has been validated and applied to the determination of renal metabolic rate in vivo. Using an indwelling detector coil, 31P NMR spectra from one kidney of anesthetized rats were quantified. The concentration of ATP was the same as that determined enzymatically, but both ADP and Pi were substantially lower. Only 25% of renal Pi and virtually none of the ADP were detected by NMR. The remainder is assumed to be bound to proteins. These concentrations of metabolites contributed to a significantly increased phosphorylation potential, which in turn should increase the free energy of hydrolysis of ATP. Saturation transfer, a non-destructive magnetic technique for the measurement of chemical exchange, was readily able to detect synthesis of ATP from ADP and inorganic phosphate. The rate of ATP synthesis determined was comparable to that determined in parallel studies of renal oxygen consumption. An ATP:O ratio of approximately 2 was found, indicating that fatty acid is the preferred fuel of respiration of the rat kidney in vivo.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·T R BrownR G Shulman
Apr 1, 1969·The Biochemical Journal·M J Weidemann, H A Krebs
Jan 1, 1984·Advances in Experimental Medicine and Biology·B D RossL Chan
Nov 15, 1984·The Biochemical Journal·M StubbsB D Ross
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A P KoretskyM W Weiner
Apr 5, 1983·Biochimica Et Biophysica Acta·D FreemanB Ross
Jan 1, 1982·The American Journal of Physiology·R A MeyerT R Brown

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Citations

Jun 1, 1990·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·N ShineM W Weiner
Nov 1, 1989·The Journal of Membrane Biology·J P Wehrle, P L Pedersen
Jun 1, 1990·Acta paediatrica Scandinavica·P F HoyerR Pichlmayr
Sep 1, 1987·The Journal of Clinical Investigation·M NakamotoR W Schrier
Nov 1, 1987·The Journal of Clinical Investigation·J I Shapiro, L Chan
Mar 1, 1991·Kidney International·A W Jans, R K Kinne
Jan 1, 1986·Kidney International·B RossL Chan
Jan 1, 1987·Annals of the New York Academy of Sciences·N ShineM W Weiner
Dec 1, 1989·Transplant International : Official Journal of the European Society for Organ Transplantation·H RuderK Schärer
Sep 1, 1991·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·C JarusiripipatR W Schrier
Oct 1, 1989·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·F TerrierF J Frey
Feb 8, 2005·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yuko OyamaAkihiko Saito
Oct 1, 1994·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·A W WilliamsD S Milliner
Jan 1, 1991·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·R B EttengerR N Fine
Dec 4, 2019·American Journal of Physiology. Renal Physiology·Aurélie EdwardsAnita T Layton
Jul 1, 1989·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·V M ReznikS A Mendoza
Oct 1, 1987·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·R G WalkerP Kincaid-Smith
Feb 15, 1990·Klinische Wochenschrift·R K KinneR W Grunewald

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