Magnetic Retrieval of Encapsulated Beta Cell Transplants from Diabetic Mice Using Dual-Function MRI Visible and Retrievable Microcapsules.

Advanced Materials
Derfogail DelcassianDaniel G Anderson

Abstract

Encapsulated beta cell transplantation offers a potential cure for a subset of diabetic patients. Once transplanted, beta cell grafts can help to restore glycemic control; however, locating and retrieving cells in the event of graft failure may pose a surgical challenge. Here, a dual-function nanoparticle-loaded hydrogel microcapsule is developed that enables graft retrieval under an applied magnetic field. Additionally, this system facilitates graft localization via magnetic resonance imaging (MRI), and graft isolation from the immune system. Iron oxide nanoparticles encapsulated within alginate hydrogel capsules containing viable islets are transplanted and the in vitro and in vivo retrieval of capsules containing nanoparticles functionalized with various ligands are compared. Capsules containing islets co-encapsulated with COOH-coated nanoparticles restore normal glycemia in immunocompetent diabetic mice for at least 6 weeks, can be visualized using MRI, and are retrievable in a magnetic field. Application of a magnetic field for 90 s via a magnetically assisted retrieval device facilitates rapid retrieval of up to 94% (±3.1%) of the transplant volume 24 h after surgical implantation. This strategy aids monitoring of cell-ca...Continue Reading

References

Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·J HuwylerW M Pardridge
Sep 29, 2006·The New England Journal of Medicine·A M James ShapiroJonathan R T Lakey
May 20, 1996·Biotechnology and Bioengineering·M S ShoichetS R Winn
Oct 2, 2009·Diabetes·David M HarlanUNKNOWN Immunology of Diabetes Society
May 4, 2010·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·B NaeyeS C De Smedt
Jun 23, 2012·Diabetes Care·Franca B BartonA M James Shapiro
Jul 21, 2012·International Journal of Pharmaceutics·Teresa Simón-YarzaMaría J Blanco-Prieto
Dec 5, 2012·Advanced Healthcare Materials·Minglin MaDaniel G Anderson
Mar 1, 2016·Acta Diabetologica·Silvia PellegriniLorenzo Piemonti
Dec 28, 2016·European Journal of Nuclear Medicine and Molecular Imaging·Andreas JodalMartin Béhé
May 20, 2017·Current Diabetes Reports·Mustafa OmamiJosé Oberholzer
Nov 11, 2017·Scientific Reports·Alexander BalhuizenNick Devoogdt
Feb 28, 2019·Proceedings of the National Academy of Sciences of the United States of America·Virginia SpanoudakiDaniel G Anderson
Dec 11, 2017·ACS Biomaterials Science & Engineering·Sary SarkisCorinne A Hoesli

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Citations

Oct 7, 2020·International Journal of Molecular Sciences·Stephane DemineDecio L Eizirik
Mar 20, 2021·Frontiers in Endocrinology·Dian R Arifin, Jeff W M Bulte
Jan 27, 2021·Stem Cells·Phillip L Lewis, James M Wells
Jun 12, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Tania B Lopez-MendezRosa Maria Hernandez
Sep 4, 2021·Nanoscale·Yizhi ZhangIsabel García

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