Magnetic-silica based nucleic acid extraction for Human Immunodeficiency Virus Type-1 drug-resistance testing in low viremic patients

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
Maria Grazia MiliaValeria Ghisetti

Abstract

Human Immunodeficiency Virus Type-1 (HIV-1) drug-resistance testing is challenging for viral loads below 1,000 copies/mL, but, according to HIV-1 guidelines, it should be considered for improving patient management and treatment options. High-recovery and high-purity extraction methods can enhance standard performances of HIV-1 genotyping assays based on direct full-population sequencing. Aim of the present study was to evaluate performances of the NucliSENS easyMAG (NeM) (BioMerieux, Marcy l'Etoile, F) semi-automated nucleic acid extraction system combined with the direct full-population sequencing ViroSeq HIV-1 genotyping (Abbott, IL, US), for detecting drug resistance in samples with HIV-1 RNA<1,000 copies/mL (n=62). Data were compared with those from the ViroSeq manual extraction in 86 samples with HIV-1 RNA<1,000 copies/mL and studied on HIV-1 reference standards. HIV-1 genotyping was successful in 98% of samples extracted with NeM (61/62) and in 84% of those extracted with ViroSeq (72/86) (X(2)=8.508, p=0.004). HIV-1 RNA levels in samples successfully processed with NeM were significantly lower than those in manually processed ones (mean+/-SD, respectively, 285+/-222 copies/mL vs. 403+/-269 copies/mL) (p=0.004). For HIV-1...Continue Reading

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Citations

Jan 17, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Maria Mercedes SantoroCarlo Federico Perno
Jul 30, 2016·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Mauro ZaccarelliAndrea Antinori
Feb 17, 2017·The Analyst·Vojtech Adam, Marketa Vaculovicova
Jan 31, 2012·Intervirology·Martin Stürmer, Claudia Reinheimer
Oct 4, 2011·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Michael J McConnellPilar Perez-Romero

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