Magnetic targeting increases mesenchymal stromal cell retention in lungs and enhances beneficial effects on pulmonary damage in experimental silicosis.

Stem Cells Translational Medicine
Luisa H A SilvaPatricia R M Rocco

Abstract

Silicosis is a pneumoconiosis caused by inhaled crystalline silica microparticles, which trigger inflammatory responses and granuloma formation in pulmonary parenchyma, thus affecting lung function. Although systemic administration of mesenchymal stromal cells (MSCs) ameliorates lung inflammation and attenuates fibrosis in experimental silicosis, it does not reverse collagen deposition and granuloma formation. In an attempt to improve the beneficial effects of MSCs, magnetic targeting (MT) has arisen as a potential means of prolonging MSC retention in the lungs. In this study, MSCs were incubated with magnetic nanoparticles and magnets were used for in vitro guidance of these magnetized MSCs and to enhance their retention in the lungs in vivo. In vitro assays indicated that MT improved MSC transmigration and expression of chemokine receptors. In vivo, animals implanted with magnets for 48 hours had significantly more magnetized MSCs in the lungs, suggesting improved MSC retention. Seven days after magnet removal, silicotic animals treated with magnetized MSCs and magnets showed significant reductions in static lung elastance, resistive pressure, and granuloma area. In conclusion, MT is a viable technique to prolong MSC retentio...Continue Reading

References

Apr 1, 1990·American Journal of Respiratory Cell and Molecular Biology·K E DriscollG Ridder
Jun 1, 1985·Journal of Applied Physiology·J H BatesJ Milic-Emili
Oct 1, 1996·American Journal of Respiratory and Critical Care Medicine·J JagirdarW N Rom
Oct 6, 1997·American Journal of Respiratory and Critical Care Medicine·E HamelmannE W Gelfand
Mar 15, 2001·Journal of Applied Physiology·D S FaffeW A Zin
Apr 1, 2008·The International Journal of Biochemistry & Cell Biology·Annika Andersson-SjölandMoisés Selman
Jun 25, 2008·Journal of Nanoscience and Nanotechnology·Erika Regina Leal de FreitasLidia Andreu Guillo
Jan 28, 2009·Nature Reviews. Immunology·Antonio UccelliVito Pistoia
Sep 19, 2009·Respiratory Physiology & Neurobiology·Roberta M LassanceMarcelo M Morales
Mar 26, 2010·Trends in Molecular Medicine·James Ankrum, Jeffrey M Karp
Apr 27, 2012·Lancet·Chi Chiu LeungWeihong Chen
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Nov 23, 2012·Chest·Daniel J WeissDonald P Tashkin
Mar 13, 2013·Regenerative Medicine·Paulo Henrique Rosado-de-CastroLea Mirian Barbosa da Fonseca
Dec 21, 2013·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Miquéias Lopes-PachecoMarcelo M Morales
Oct 3, 2014·Respiratory Research·Mariana A AntunesPatricia R M Rocco
Dec 23, 2014·The Lancet. Respiratory Medicine·Jennifer G WilsonMichael A Matthay
Jul 3, 2015·Journal of Functional Biomaterials·Jhon CoresKe Cheng
Sep 22, 2015·Regenerative Medicine·John J ConnellTammy L Kalber
Jan 21, 2016·PloS One·Fernanda Ferreira CruzPatricia Rieken Macedo Rocco
Jan 29, 2016·QJM : Monthly Journal of the Association of Physicians·Jan StolkPieter S Hiemstra
Apr 12, 2016·Stem Cells International·Miquéias Lopes-PachecoMarcelo M Morales
Sep 2, 2016·Stem Cell Research & Therapy·Jeffrey L SpeesCarl A Gregory
Mar 11, 2017·Stem Cell Research & Therapy·Luisa H A SilvaPatricia R M Rocco
Mar 21, 2017·Stem Cells·Franziska NitzscheJohannes Boltze
Nov 12, 2017·Stem Cell Research & Therapy·Helena D'Anunciação de OliveiraMarcelo Marcos Morales
Feb 28, 2018·Stem Cell Research & Therapy·Luisa H A SilvaPatricia R M Rocco
Mar 30, 2018·International Journal of Nanomedicine·Sylwia DabrowskaBarbara Lukomska
Jun 23, 2018·Nanomedicine : Nanotechnology, Biology, and Medicine·Luisa H A SilvaPatricia R M Rocco
Jan 23, 2019·International Journal of Nanomedicine·Xiuying LiJinlan Jiang

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Citations

Jul 28, 2021·Expert Opinion on Drug Delivery·Yukiya TakayamaMakiya Nishikawa
Nov 7, 2021·Small·Miriam FilippiRobert K Katzschmann

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Methods Mentioned

BETA
genetic modification
atomic absorption
transmission electron microscopy
flow cytometry
bronchoalveolar
lavage
PCR
fluorescence imaging
Fluorescence
transfection

Software Mentioned

GraphPad
Flowing
ImageJ
Living Image
GraphPad Prism

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