Magnetically Assisted Surface-Enhanced Raman Spectroscopy for the Detection of Staphylococcus aureus Based on Aptamer Recognition

ACS Applied Materials & Interfaces
Junfeng WangShengqi Wang

Abstract

A magnetically assisted surface-enhanced Raman scattering (SERS) biosensor for single-cell detection of S. aureus on the basis of aptamer recognition is reported for the first time. The biosensor consists of two basic elements including a SERS substrate (Ag-coated magnetic nanoparticles, AgMNPs) and a novel SERS tag (AuNR-DTNB@Ag-DTNB core-shell plasmonic NPs or DTNB-labeled inside-and-outside plasmonic NPs, DioPNPs). Uniform, monodisperse, and superparamagnetic AgMNPs with favorable SERS activity and magnetic responsiveness are synthesized by using polymer polyethylenimine. AgMNPs use magnetic enrichment instead of repeated centrifugation to prevent sample sedimentation. DioPNPs are designed and synthesized as a novel SERS tag. The Raman signal of DioPNPs is 10 times stronger than that of the commonly used SERS tag AuNR-DTNB because of the double-layer DTNB and the LSPR position adjustment to match the given laser excitation wavelength. Consequently, a strong SERS enhancement is achieved. Under the optimized aptamer density and linker length, capture by aptamer-modified AgMNPs can achieve favorable bacteria arrest (up to 75%). With the conventional Raman spectroscopy, the limit of detection (LOD) is 10 cells/mL for S. aureus d...Continue Reading

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Citations

Aug 16, 2016·Nanomedicine : Nanotechnology, Biology, and Medicine·Rui XiaoShengqi Wang
Sep 28, 2016·Frontiers in Microbiology·Jun TengBaoguang Li
Nov 14, 2018·Artificial Cells, Nanomedicine, and Biotechnology·Ravichandran MLeija L
Jul 28, 2020·International Journal of Molecular Sciences·Nevina E Trunzo, Ka Lok Hong
Feb 6, 2020·Proceedings of the National Academy of Sciences of the United States of America·Wei ZhangAnhong Zhou
Jun 14, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Chuyue ZhangXiangmei Chen
Sep 28, 2018·Nanomaterials·Shuyuan DuLixia Lu
Dec 12, 2020·Advanced Science·Xia ZhouPinghua Sun
Mar 1, 2018·Comprehensive Reviews in Food Science and Food Safety·Kaiqiang WangDa-Wen Sun
Mar 15, 2021·Talanta·Muhammad Muhammad, Qing Huang
May 4, 2021·Talanta·Elham SheikhzadehMohammed Zourob
Jul 30, 2021·Analytical and Bioanalytical Chemistry·Yi-Wei WengXiao-Lan Wei
Jun 1, 2019·ACS Applied Materials & Interfaces·Dongmei LvHongsheng Ouyang

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