Magnetism-Resolved Separation and Fluorescence Quantification for Near-Simultaneous Detection of Multiple Pathogens

Analytical Chemistry
Linyao LiWeihong Tan

Abstract

In the modern era of molecular evidence-based medicine and advanced biomedical technologies, the rapid, sensitive and specific assay of multiple pathogens is critical to, but largely absent from, clinical practice. Therefore, to improve the current ordinary separation and collection method, we report herein a strategy of magnetism-resolved separation and fluorescence quantification for near-simultaneous detection of multiple pathogens, followed by the direct antimicrobial susceptibility testing (AST). To accomplish this strategy, we utilized aptamer-modified fluorescent-magnetic multifunctional nanoprobes (apt-FMNPs). FMNPs with intriguing different magnetic responses and excellent fluorescence quality were first self-assembled based on metal coordination interaction using (3-mercaptopropyl) trimethoxysilane, magnetic γ-Fe2O3, and fluorescent quantum dots as matrix components. Then, aptamers, which specific to target pathogens of Escherichia coli O157:H7 ( E. coli) and Salmonella typhimurium ( S. typ), were conjugated with FMNPs to yield apt-FMNPs nanoprobes for multiple pathogens assay. Based on the discrepant magnetic response of pathogen@nanoprobes complex under the identical external magnetic field, the model bacteria were ...Continue Reading

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Citations

Jul 13, 2019·Critical Reviews in Food Science and Nutrition·Wei WuQingli Yang
Jul 7, 2020·Emerging Microbes & Infections·Hui-Yan LiJian Wu
Feb 9, 2021·World Journal of Microbiology & Biotechnology·Danliang LiLili Chen
Feb 14, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Clara I ColinoCarmen Gutierrez-Millan
Jan 15, 2021·Comprehensive Reviews in Food Science and Food Safety·Yafang ShenYanbin Li
May 11, 2021·ACS Applied Materials & Interfaces·Yi-Yan BaiZhi-Ling Zhang
Apr 20, 2021·Annual Review of Biomedical Engineering·Lia A StanciuNoor Mohammad
Jul 21, 2021·Critical Reviews in Food Science and Nutrition·Yi XuQuansheng Chen

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