Magnetization transfer in protein solutions at 0.1 T: dependence on concentration, molecular weight, and structure

Academic Radiology
A VirtaN Lundbom

Abstract

We observed the magnetization transfer rates in a variety of protein solutions at 0.1-T magnetic field and compared our results with previous investigations at high magnetic fields (> 0.5 T). The effects of protein concentration, size, pH, denaturation, cross linking, and fiber formation were investigated. We used the saturation transfer technique to determine the transfer of magnetization in gamma globulin, fibronectin, collagen, fibrinogen, and albumin solutions. The observed transfer rate increased with increasing concentration and size of the protein. Protein degradation decreased the transfer rate. Cross linking and fiber formation each increased the transfer rate, whereas buffer pH had no effect. Protein denaturation, aggregation, and fiber formation are important determinants of magnetization transfer in vitro. The size, concentration, and cross linking of the proteins contribute strongly to the transfer of magnetization at low fields, and the effect seems to be at least as important as at the higher fields.

References

Jul 12, 1979·The New England Journal of Medicine·D J ProckopN A Guzman
Jul 5, 1979·The New England Journal of Medicine·D J ProckopN A Guzman
Jan 1, 1992·Journal of Computer Assisted Tomography·J V HajnalG M Bydder
Sep 1, 1991·Radiology·R S BalabanF W Heineman
Jun 1, 1991·Radiology·S D WolffR S Balaban
Feb 1, 1991·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·J GradR G Bryant
Mar 1, 1991·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·T A FralixR S Balaban
Feb 1, 1990·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·J H ZhongI M Armitage
Aug 1, 1990·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·M RydzyJ K Saunders
Apr 1, 1989·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·S D Wolff, R S Balaban
Jul 1, 1988·Investigative Radiology·S H Koenig, R D Brown
Apr 1, 1995·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·C Morrison, R M Henkelman
Dec 1, 1994·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·D Zhou, R G Bryant
Oct 1, 1994·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·M Iino
Mar 1, 1994·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·D BrooksT Schleich
Dec 1, 1993·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·S H Koenig, R D Brown
Jun 1, 1993·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·R M HenkelmanM J Bronskill
Mar 1, 1993·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·S H KoenigR Ugolini
Feb 1, 1993·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·D K KimR S Balaban

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Citations

Nov 6, 1998·Magnetic Resonance Imaging·L D HallK P Nott
Apr 9, 1999·Magnetic Resonance Imaging·K P NottL D Hall
Aug 27, 1999·Magnetic Resonance Imaging·S K KoskinenM E Komu
Jun 2, 2011·American Journal of Veterinary Research·Géraldine E BolenValeria Busoni

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