Magneto-optic spectroscopy of a protein tetramer binding two exciton-coupled chlorophylls

Journal of the American Chemical Society
Joseph L HughesElmars Krausz

Abstract

In vitro chlorophyll (Chl) aggregates have often served as models for in vivo forms of long-wavelength Chl. However, the interaction of protein-bound Chl molecules is typically different than that occurring in solvent-based self-aggregates. We have chosen a water-soluble Chl-binding protein (WSCP) from cauliflower in order to help characterize the spectroscopic properties of Chl in a single well-defined native environment and also to study the pigment-pigment (exciton) interactions present in assemblies of this protein. WSCP forms tetrameric units upon binding two Chl molecules. We present the absorption, circular dichroism (CD), magnetic circular dichroism (MCD), and emission spectra at 1.7 K of recombinant WSCP tetramers containing either Chl a or Chl d. The spectroscopic characteristics provide evidence for significant exciton interaction between equivalent Chl molecules. Our simple exciton analysis allows an estimate of the molecular geometry of the dimer, which is predicted to have an "open sandwich"-type structure. We find that the ratio of the magnetic circular dichroism to absorption, deltaA/A, is substantially increased (approximately 60%) for Chl a in this system compared to its value in solution. This increase is in ...Continue Reading

References

Dec 8, 1976·Journal of the American Chemical Society·L L ShipmanJ J Katz
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·R BassiD Sandonà
Dec 26, 2001·Biophysical Journal·Giuseppe ZucchelliOliviero Cremonesi
Dec 4, 2002·Biochimica Et Biophysica Acta·Teodor Silviu BalabanValentin I Prokhorenko
Dec 4, 2003·Nature·David StroebelDaniel Picot
Aug 17, 2005·Biochemistry·M Reza RazeghifardTom Wydrzynski
Oct 18, 2005·Photosynthesis Research·J P Dekker, R Van Grondelle
Oct 26, 2005·Photosynthesis Research·Leszek FiedorKazimierz Strzałka
Feb 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·C E Strouse
Mar 1, 1984·Biophysical Chemistry·D Zevenhuijzen, P J Zandstra

❮ Previous
Next ❯

Citations

Oct 24, 2007·Photosynthesis Research·Tom WydrzynskiBrendon Conlan
Sep 5, 2008·Photosynthesis Research· Govindjee
Sep 9, 2008·Photosynthesis Research·Brendon Conlan
Oct 3, 2008·Photosynthesis Research·Reza Razeghifard
Jun 1, 2010·Photosynthesis Research·Adele WilliamsonTom Wydrzynski
Jul 11, 2013·Photosynthesis Research·Elmars Krausz
Dec 23, 2009·The Journal of Chemical Physics·Mike ReppertRyszard Jankowiak
Jan 27, 2016·Journal of the American Chemical Society·Julian AdolphsThomas Renger
Oct 22, 2014·Bioscience, Biotechnology, and Biochemistry·Shigekazu TakahashiHiroyuki Satoh
Feb 26, 2016·Photochemistry and Photobiology·Adam KellRyszard Jankowiak
Mar 3, 2017·Biochemistry·Daniel M PalmHarald Paulsen
Apr 28, 2020·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Philipp GirrHarald Paulsen
Dec 16, 2006·The Journal of Biological Chemistry·Daisuke HorigomeAkira Uchida
Nov 28, 2018·Photosynthesis Research·Brendon ConlanJohannes Messinger
Mar 19, 2014·The Journal of Physical Chemistry. B·Jan AlsterDonatas Zigmantas
Oct 6, 2011·The Journal of Physical Chemistry. a·M Belén Oviedo, Cristián G Sánchez
Aug 27, 2021·The Journal of Physical Chemistry. a·Ashish SharmaGirish Lakhwani

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.