Magnetoresistive-based real-time cell phagocytosis monitoring

Biosensors & Bioelectronics
A ShoshiH Brueckl

Abstract

The uptake of large particles by cells (phagocytosis) is an important factor in cell biology and also plays a major role in biomedical applications. So far, most methods for determining the phagocytic properties rely on cell-culture incubation and end-point detection schemes. Here, we present a lab-on-a-chip system for real-time monitoring of magnetic particle uptake by human fibroblast (NHDF) cells. It is based on recording the time evolution of the average position and distribution of magnetic particles during phagocytosis by giant-magnetoresistive (GMR) type sensors. We employ particles with a mean diameter of 1.2 μm and characterize their phagocytosis-relevant properties. Our experiments at physiological conditions reveal a cellular uptake rate of 45 particles per hour and show that phagocytosis reaches saturation after an average uptake time of 27.7h. Moreover, reference phagocytosis experiments at 4°C are carried out to mimic environmental or disease related inhibition of the phagocytic behavior, and our measurements clearly show that we are able to distinguish between cell-membrane adherent and phagocytosed magnetic particles. Besides the demonstrated real-time monitoring of phagocytosis mechanisms, additional nano-bioin...Continue Reading

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Citations

Apr 15, 2014·Trends in Biotechnology·Peter ErtlGünter Lepperdinger
Jul 3, 2015·Biotechnology Advances·Mario RothbauerPeter Ertl
Dec 14, 2018·Nanomaterials·Soyeon JeonWan-Seob Cho

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