Magnetosomes and magnetite crystals produced by magnetotactic bacteria as resolved by atomic force microscopy and transmission electron microscopy

Micron : the International Research and Review Journal for Microscopy
Zachery OestreicherBrian H Lower

Abstract

Atomic force microscopy (AFM) was used in concert with transmission electron microscopy (TEM) to image magnetotactic bacteria (Magnetospirillum gryphiswaldense MSR-1 and Magnetospirillum magneticum AMB-1), magnetosomes, and purified Mms6 proteins. Mms6 is a protein that is associated with magnetosomes in M. magneticum AMB-1 and is believed to control the synthesis of magnetite (Fe(3)O(4)) within the magnetosome. We demonstrated how AFM can be used to capture high-resolution images of live bacteria and achieved nanometer resolution when imaging Mms6 protein molecules on magnetite. We used AFM to acquire simultaneous topography and amplitude images of cells that were combined to provide a three-dimensional reconstructed image of M. gryphiswaldense MSR-1. TEM was used in combination with AFM to image M. gryphiswaldense MSR-1 and magnetite-containing magnetosomes that were isolated from the bacteria. AFM provided information, such as size, location and morphology, which was complementary to the TEM images.

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·E W SilvertonD R Davies
Feb 10, 2004·Applied and Environmental Microbiology·Karen GrünbergDirk Schüler
Mar 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Arash KomeiliDianne K Newman
Apr 15, 2004·Nature Reviews. Microbiology·Dennis A Bazylinski, Richard B Frankel
May 15, 2004·EMBO Reports·Ferry KienbergerPeter Hinterdorfer
Aug 2, 2005·Trends in Microbiology·Katrina J EdwardsThomas M McCollom
Sep 19, 2006·Nature Reviews. Microbiology·Karrie A WeberJohn D Coates
Mar 22, 2008·Science·Michael F HochellaBenjamin S Twining
May 5, 2010·Proceedings of the National Academy of Sciences of the United States of America·Daisuke YamamotoYoshihiro Fukumori
Feb 22, 2011·Current Opinion in Biotechnology·Eugene L Madsen

❮ Previous
Next ❯

Citations

Oct 13, 2015·Protein Science : a Publication of the Protein Society·Shiran Barber-ZuckerRaz Zarivach
Jul 9, 2013·International Journal of Pharmaceutics·Alexandru Mihai GrumezescuCarmen Mariana Chifiriuc
Jun 6, 2016·World Journal of Microbiology & Biotechnology·T RevathyK Suthindhiran
Jun 3, 2017·3 Biotech·T RevathyK Suthindhiran
Aug 16, 2021·Micron : the International Research and Review Journal for Microscopy·Nadia N Casillas-ItuarteSteven K Lower

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.