Magnitude, breadth, and functional profile of T-cell responses during human immunodeficiency virus primary infection with B and BF viral variants

Journal of Virology
Gabriela TurkHoracio Salomon

Abstract

The molecular pattern of the human immunodeficiency virus (HIV) epidemic in Argentina provides an appropriate scenario to study cellular immune responses in patients with non-clade B infection. We aimed to map T-cell responses in patients infected with BF recombinant variants and compare them with those of clade B patients. Sixteen recently infected patients were enrolled and grouped by viral subtype. Nef-specific responses were evaluated with a peptide matrix-based gamma interferon (IFN-gamma) enzyme-linked immunospot (ELISPOT) assay using B and BF overlapping peptides. Cross-clade and clade-specific responses were found. A correlation between B versus BF Nef-specific responses was identified. Detailed analysis at the single-peptide level revealed that BF patients show a narrower response but greater magnitude. Nef immunodominant responses agreed with previous publications, although the B loop was targeted at an unexpectedly high frequency. The putative HLA allele(s) restricting each positive response was determined. Single-peptide level screening with two different peptide sets uncovered discordant responses (mostly caused by peptide offsetting) and allowed detection of increased breadth. Positive responses identified by ELIS...Continue Reading

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Citations

Sep 18, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Miguel-Angel PeralesJedd D Wolchok
Oct 25, 2008·Annual Review of Medicine·Daniel C DouekRichard A Koup
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Mar 18, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Victor Y DuPaul A Goepfert

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