PMID: 7546Jun 1, 1976

Magnitude of the protonmotive force in respiring Staphylococcus aureus and Escherichia coli

Journal of Bacteriology
S H Collins, W A Hamilton

Abstract

The membrane potential and pH gradient developed across the plasma membranes of whole cells of Staphylococcus aureus and spheroplasts of Escherichia coli were estimated. The distributions of potassium ions in the presence of valinomycin and the pH gradient across the membrane were determined from the changes in pK and pH observed in the external medium during transition from the energized respiring state to the de-engerized resting condition. The protonmotive force in respiring cells was estimated at 211 mV for S. aureus and 230 mV for E. coli at external pH values of approximately 6.5. The adequacy of these protonmotive forces as a driving force for substrate accumulation or adenosine 5'-triphosphate synthesis is discussed.

Citations

Jun 13, 2001·Canadian Journal of Microbiology·D F Niven, A Ekins
May 1, 1981·European Journal of Biochemistry·K NicolayW N Konings
Nov 22, 2013·Journal of the Royal Society, Interface·Marcel ReuterIan R Booth
Feb 18, 2020·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Christian WölkRichard D Harvey
Jan 1, 1977·Journal of Supramolecular Structure·L W AdlerB P Rosen

Related Concepts

Striadyne
Plasma Membrane
Respiratory Chain
Alkalescens-Dispar Group
Hydrogen-Ion Concentration
Resting Potentials
Oxygen Consumption
Potassium
Spheroplasts
Staphylococcus aureus

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