Magnoflorine with hyaluronic acid gel promotes subchondral bone regeneration and attenuates cartilage degeneration in early osteoarthritis

Bone
Zhe CaiKwong-Yuen Chiu

Abstract

To investigate efficacy of Chinese medicine magnoflorine combined with hyaluronic acid (HA)-gel in promoting subchondral bone (SCB) regeneration and attenuating cartilage degeneration in early osteoarthritis (OA). MC3T3-E1 under magnoflorine treatment was assayed by XTT to determine cell viability. Cell proliferation was reflected through cell cycle. Osteoblast mineralization was stained by Alizarin Red. Standardized bone canal of 1 mm in diameter and 4 mm in depth was made on tibial medial plateau of 4-month-old Dunkin-Hartley spontaneous knee OA guinea pigs. Guinea pigs (n = 5/group) were treated once intra-bone canal injection of 2 μl HA-gel, 2 μl HA-gel+50 ng magnoflorine and null (Defect) respectively, except sham group. The left hind limbs were harvested for μCT scan and histopathological staining 2-month post-surgery. 25 μg/ml magnoflorine treatment significantly increased cell viability, S-phase and mineralization of MC3T3-E1 cells. In vivo, HA-gel + magnoflorine treatment significantly altered SCB microstructure; changes included increase in bone volume fraction (BV/TV), trabecular number (Tb.N), connectivity density (Conn.Dn), and decrease in degree of anisotropy (DA), which implied trabecular bone regeneration. Treat...Continue Reading

Citations

Apr 28, 2020·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·Yueping JiangShao Liu
Apr 28, 2020·BioMed Research International·Xuchang ZhouWei Wu
Feb 10, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Aihua HuangHui Xu
Dec 7, 2020·Journal of Experimental Orthopaedics·Elisha JohnstonDavid Rabago
Jan 9, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Tong XuGang Fan
Feb 9, 2021·Frontiers in Cell and Developmental Biology·Xiaobo ZhuYangzi Jiang
Feb 27, 2021·Clinical and Translational Medicine·Zhe CaiHuasong Zeng

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