Magnolol inhibits Mac-1 (CD11b/CD18)-dependent neutrophil adhesion: relationship with its antioxidant effect

European Journal of Pharmacology
Y C ShenC F Chen

Abstract

Magnolol, a phenolic compound isolated from a Chinese herbal drug, Magnolia officinalis, has been shown to protect rat heart from ischemia/reperfusion injury. Neutrophil adhesion plays a crucial process during this inflammatory response. To evaluate whether magnolol prevents ischemia/reperfusion injury by inhibiting neutrophil adhesion, we determined whether magnolol can inhibit adhesion of phorbol-12-myristate-13-acetate (PMA)-activated human neutrophils to a fibrinogen-coated surface in a dose-dependent manner. Using flow cytometric analysis, we observed that magnolol pretreatment (10 min at 37 degrees C) diminished PMA (100 ng/ml)-induced Mac-1 upregulation. PMA also induced rapid intracellular accumulation of superoxide (O2-.) and hydrogen peroxide (H2O2) in neutrophils; magnolol pretreatment attenuated the accumulation of these two substances. Inhibition of reactive oxygen species by superoxide dismutase and/or catalase, which decompose O2-. and H2O2, respectively, also abolished Mac-1 upregulation and neutrophil adhesion. We conclude that magnolol inhibits neutrophil adhesion and that this can account for its anti-ischemia/reperfusion injury effect. We propose that the inhibitory effect of magnolol on neutrophil adhesion ...Continue Reading

References

Dec 1, 1992·Naunyn-Schmiedeberg's Archives of Pharmacology·J P WangC M Teng
Apr 1, 1990·The Journal of Experimental Medicine·J F BohnsackG A Zimmerman
Aug 2, 1990·Nature·T A Springer
Jan 1, 1991·The Journal of Clinical Investigation·E Ruoslahti
Jun 15, 1988·Thrombosis Research·C M TengH Y Hsu
Sep 1, 1995·The Annals of Thoracic Surgery·A M Lefer
Feb 1, 1993·The Journal of Clinical Investigation·M P Bevilacqua, R M Nelson
Jan 1, 1994·Methods in Cell Biology·J P RobinsonP K Narayanan
Aug 1, 1996·Journal of Leukocyte Biology·E A EverittB Hendey
Aug 23, 1996·Biochimica Et Biophysica Acta·C V SerranoJ L Zweier
Aug 1, 1996·Toxicology and Applied Pharmacology·H JaeschkeR A Roth
Aug 1, 1996·Clinical and Experimental Pharmacology & Physiology·C Y HongS K Tsai

❮ Previous
Next ❯

Citations

Mar 26, 2003·European Journal of Pharmacology·Yuh-Chiang ShenChieh-Fu Chen
Feb 11, 2009·Antioxidants & Redox Signaling·Levi E Fried, Jack L Arbiser
Apr 23, 2005·Neurological Research·Hocheol Kim
Aug 2, 2012·Journal of Biomedical Science·Jennifer Hui-Chun Ho, Chuang-Ye Hong
Apr 6, 2006·Biological & Pharmaceutical Bulletin·Zhao Xiang BianMin Li
Nov 3, 2009·Pulmonary Pharmacology & Therapeutics·Karin J VelthoveWouter W van Solinge
Mar 8, 2006·British Journal of Pharmacology·Shih-Chung ChenJing-Jy Cheng
Jan 25, 2003·The Journal of Pharmacy and Pharmacology·Yvonne WangMing-Kuan Hu
Dec 13, 2005·Journal of Pharmacological Sciences·Chieh-Hsi WuJui-Sung Hung
Aug 28, 2016·Fundamental & Clinical Pharmacology·Lei LiMenbayaer Tu
Aug 6, 2009·The American Journal of Chinese Medicine·Ke YaoYi-Dong Zhang
Oct 12, 2004·The International Journal of Biochemistry & Cell Biology·Wang-Fun FongCheng Wang
Dec 18, 2008·Journal of Natural Products·Chien-Chang ShenChien-Chih Chen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.