Maid: a maternally transcribed novel gene encoding a potential negative regulator of bHLH proteins in the mouse egg and zygote

Developmental Dynamics : an Official Publication of the American Association of Anatomists
S Y HwangBarbara B Knowles

Abstract

We isolated an abundant novel cDNA SSEC-8 from a subtraction cDNA library enriched for maternal transcripts that are still present in the mouse 2 cell stage embryo. This gene is evolutionarily conserved and maps to the distal region of mouse chromosome 2. The deduced polypeptide sequence of the encoded protein contains a conserved helix-loop-helix (HLH) motif without a basic DNA binding domain, suggesting that it functions as a negative regulator of basic (b) HLH transcription factors. Gel mobility shift assays show that in vitro translated protein prevents the E12/MyoD bHLH dimer from binding to DNA. Also, transient overexpression of this protein in C2C12 cells reduced the transcription of a CAT-reporter regulated by an E12/MyoD driven enhancer. The 3'-UTR contains consensus sequences of cytoplasmic polyadenylation elements (CPE's), and the length of its poly (A) tail changes during oocyte maturation, indicating that its expression is controlled by timely activation of translation. This new gene, Maid, models the translational and transcriptional regulation of gene expression during the transition from gamete to embryo.

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Citations

Dec 19, 2000·Biochemical and Biophysical Research Communications·M S ChangY C Yang
May 24, 2015·Journal of Biochemistry·Mitsuyoshi MotizukiKeiji Miyazawa
Apr 14, 2009·Molecular Reproduction and Development·Alexei V Evsikov, Caralina Marín de Evsikova
Mar 21, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Benjamin N HarwoodWilhelmine N De Vries
Sep 20, 2005·Biochemical and Biophysical Research Communications·Takayuki SakuraiMinoru Kimura
Aug 1, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S TeraiS S Thorgeirsson
May 29, 2016·Journal of Cell Science·Yan HuangWenbin Ma
Jul 5, 2018·FEBS Letters·Cecilia Lanny Winata, Vladimir Korzh
Jun 5, 2001·Molecular Reproduction and Development·S Y HwangJ S Lee
Nov 30, 2006·Journal of Cellular Biochemistry·Wenbin MaMingyao Liu
Jan 30, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Eva Sonnenberg-RiethmacherDieter Riethmacher
Jul 27, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·B S HeyerD Solter
Jun 5, 1999·Microbiology and Molecular Biology Reviews : MMBR·J D Richter
May 12, 2005·Gastroenterology·Taro TakamiKiwamu Okita

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