Main-chain dynamics of cardiotoxin II from Taiwan cobra (Naja naja atra) as studied by carbon-13 NMR at natural abundance: delineation of the role of functionally important residues

Biochemistry
C S LeeC Yu

Abstract

Cardiotoxin analogue II (CTX II) is an all beta-sheet, small molecular mass (6.8 kDa), basic protein possessing a wide array of biological properties. Nearly complete assignment of the protonated carbon resonances has been achieved by heteronuclear NMR experiments. The study shows that the correlation between the carbon-13 chemical shifts and CTX II structure is good in general, but interesting deviations are also noticed. To characterize the internal dynamics of CTX II, longitudinal, transverse relaxation rates and heteronuclear 13C{1H} NOEs were measured for alpha-carbons at natural abundance by two-dimensional NMR spectroscopy. Relaxation measurements were obtained in a 14.1 T spectrometer for 50 residues, which are evenly spread along the CTX II polypeptide chain. Except for five alpha-carbons, all data were analyzed from a simple two-parameter spectral density function using the model free approach of Lipari and Szabo. The microdynamical parameters (S2, taue, and Rex) were calculated with an overall rotational correlation time (taum) for the protein of 4.8 ns. For most residues, the alpha-carbons exhibit fast (taue < 30 ps) restricted libration motions (S2 = 0.79-0.89). The present study reveals that the functionally impor...Continue Reading

References

Nov 20, 1991·Journal of Molecular Biology·D S WishartF M Richards
Jan 15, 1997·The Biochemical Journal·T SivaramanC Yu

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Citations

Nov 14, 2003·Toxicology and Applied Pharmacology·Shu-Hui SuKee-Lung Chang
Mar 10, 2000·Toxicon : Official Journal of the International Society on Toxinology·L S ChangS R Lin
Apr 23, 2002·Protein Science : a Publication of the Protein Society·Alphonse I ArunkumarChin Yu
Apr 30, 2003·Protein Science : a Publication of the Protein Society·Djaudat IdiyatullinKevin H Mayo
Oct 4, 2012·Protein Science : a Publication of the Protein Society·Chun-Ho ChengWoei-Jer Chuang
Aug 11, 2006·Toxicon : Official Journal of the International Society on Toxinology·Long-Sen ChangHsien-Bin Huang
Jun 25, 2002·Biophysical Journal·Roman G EfremovAlexander S Arseniev

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