Main histological parameters to be evaluated in an experimental model of myocardial infarct treated by stem cells on pigs

PeerJ
Soledad García Gómez-HerasMariano García-Arranz

Abstract

Myocardial infarction has been carefully studied in numerous experimental models. Most of these models are based on electrophysiological and functional data, and pay less attention to histological discoveries. During the last decade, treatment using advanced therapies, mainly cell therapy, has prevailed from among all the options to be studied for treating myocardial infarction. In our study we wanted to show the fundamental histological parameters to be evaluated during the development of an infarction on an experimental model as well as treatment with mesenchymal stem cells derived from adipose tissue applied intra-lesionally. The fundamental parameters to study in infarcted tissue at the histological level are the cells involved in the inflammatory process (lymphocytes, macrophages and M2, neutrophils, mast cells and plasma cells), neovascularization processes (capillaries and arterioles) and cardiac cells (cardiomyocytes and Purkinje fibers). In our study, we used intramyocardial injection of mesenchymal stem cells into the myocardial infarction area 1 hour after arterial occlusion and allowed 1 month of evolution before analyzing the modifications on the normal tissue inflammatory infiltrate. Acute inflammation was shorten...Continue Reading

References

Oct 9, 1998·Cardiovascular Research·P D VerdouwD J Duncker
Mar 4, 2000·The New England Journal of Medicine·S H LeeP A Thistlethwaite
Apr 1, 1957·American Heart Journal·J LITVAKA M VINEBERG
Oct 21, 2004·Blood·Sudeepta Aggarwal, Mark F Pittenger
Mar 22, 2005·Experimental Cell Research·Ida RasmussonKatarina Le Blanc
Dec 26, 2006·Cardiovascular Research·Rainer KlockeSigrid Nikol
Mar 28, 2007·Scandinavian Journal of Immunology·I RasmussonO Ringdén
Oct 8, 2009·Circulation. Heart Failure·Jennifer A Dixon, Francis G Spinale
Aug 26, 2010·Cardiovascular Research·Scott P LevickJoseph S Janicki
Sep 14, 2010·Expert Opinion on Biological Therapy·Ignacio García-GómezDamián García-Olmo
Sep 28, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Hongxia LiMeifeng Xu
Sep 13, 2011·The American Journal of Pathology·Zengxuan NongJ Geoffrey Pickering
Nov 23, 2011·Current Pharmaceutical Design·Alicia C ReidRoberto Levi
Jun 13, 2012·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Tihomir Georgiev-HristovDamián García-Olmo
Dec 12, 2012·Clinical & Developmental Immunology·Yur-Ren KuoChao-Long Chen
May 13, 2014·Cell Transplantation·Shiu-Huey ChouChih-Yang Huang
Sep 11, 2014·Journal of Cardiovascular Translational Research·Lina ChenJianguo Xu
Feb 15, 2015·Journal of Molecular and Cellular Cardiology·Joe WuGary Wright
Apr 23, 2015·IUBMB Life·Agata SzadeJozef Dulak
Oct 23, 2015·Oxidative Medicine and Cellular Longevity·W H Davin Townley-TilsonLiang Xie
Apr 28, 2016·Seminars in Cell & Developmental Biology·Jonathan LeorTal Konfino
Sep 17, 2016·Circulation Research·Bijun Chen, Nikolaos G Frangogiannis
Feb 9, 2017·Journal of Biomedical Science·Aleksandra GombozhapovaVyacheslav Ryabov
Feb 24, 2017·Methods in Molecular Biology·Taylor A Johnson, Dinender K Singla
Sep 5, 2017·Laboratory Investigation; a Journal of Technical Methods and Pathology·Akon HiguchiKadarkarai Murugan
Sep 7, 2017·Journal of Korean Medical Science·Min Chul KimYoungkeun Ahn
Oct 19, 2017·The Journal of Gene Medicine·Ayman El-Sayed ShafeiAmal S El-Shal

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence microscopy

Related Concepts

Related Feeds

Cardiac Regeneration

Cardiac regeneration enables the repair of irreversibly damaged heart tissue using cutting-edge science, including stem cell and cell-free therapy. Discover the latest research on cardiac regeneration here.