Maintaining and Monitoring the Defined Microbiota Status of Gnotobiotic Rodents

ILAR Journal
Werner NicklasAndré Bleich

Abstract

Gnotobiotic (germfree, defined colonized) rodents have become powerful tools to advance our understanding of the host-microbiome relationship. However, the maintenance and ultimately the monitoring of gnotobiotic rodents is a critical, labor-intensive, and costly process (e.g., sterility, not absence of specific pathogens, must be demonstrated in germfree animals). Here, we provide information on the housing and maintenance of gnotobiotic animals, elucidate prophylactic measurements to avoid contamination, and make specific recommendations for sampling procedures, sampling frequencies, and test methods.

References

Oct 1, 1978·Laboratory Animals·G J Moore, P Aldred
Mar 1, 1971·The Journal of Hygiene·D van der Waaij, A H Andreas
Jan 1, 1981·Annual Review of Nutrition·B S Wostmann
Mar 6, 1999·Experimental Animals·M Okamoto, T Matsumoto
Jul 31, 1999·Applied and Environmental Microbiology·F E DewhirstJ G Fox
Sep 5, 1958·Annals of the New York Academy of Sciences·J A REYNIERS, M R SACKSTEDER
May 8, 1959·Annals of the New York Academy of Sciences·B E GUSTAFSSON
May 1, 1960·The Anatomical Record·H A GORDON, B S WOSTMANN
Jul 1, 1965·The Journal of Experimental Medicine·R W SCHAEDLERR COSTELLO
Jul 1, 1965·The Journal of Experimental Medicine·R DUBOSP HOET
Jul 1, 1965·The Journal of Experimental Medicine·R W SCHAEDLERR COSTELLO
May 8, 1959·Annals of the New York Academy of Sciences·J R PLEASANTS
Nov 3, 2004·Transgenic Research·Margaret L Van Keuren, Thomas L Saunders
Oct 4, 2005·Laboratory Animals·José InzunzaLars Ahrlund-Richter
Oct 19, 2007·Nature·Peter J TurnbaughJeffrey I Gordon
Feb 5, 2008·Gastroenterology·R Balfour Sartor
Feb 19, 2008·Trends in Microbiology·Bärbel Stecher, Wolf-Dietrich Hardt
Apr 4, 2009·Nature Reviews. Immunology·June L Round, Sarkis K Mazmanian
Sep 25, 2010·Nature Reviews. Immunology·Nadine Cerf-Bensussan, Valérie Gaboriau-Routhiau
Nov 9, 2010·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·K M NeufeldJ A Foster
Nov 1, 2011·Laboratory Animals·Lydia M Janus, Andre Bleich
Jan 20, 2012·Comparative Immunology, Microbiology and Infectious Diseases·André Bleich, Axel Kornerup Hansen
Jan 31, 2012·Applied and Environmental Microbiology·Merritt G GillillandGary B Huffnagle
Jun 8, 2012·Science·Jeremy K NicholsonSven Pettersson
Apr 9, 2013·Nature Biotechnology
Jul 28, 2013·Gut Microbes·Christopher D PackeyR Balfour Sartor
Jan 24, 2014·Science Translational Medicine·Jeremiah J FaithJeffrey I Gordon
Feb 6, 2014·Laboratory Animals·UNKNOWN FELASA working group on revision of guidelines for health monitoring of rodents and rabbitsM Raspa
Oct 7, 2014·Cell·Henning SeedorfJeffrey I Gordon

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Citations

Apr 9, 2017·The Journal of Biological Chemistry·Kristina B MartinezEugene B Chang
Dec 10, 2016·Laboratory Animals·I N Vatsos
May 18, 2019·Laboratory Animals·Marijana Basic, André Bleich
Sep 2, 2015·ILAR Journal·André Bleich, James G Fox
Oct 2, 2019·Frontiers in Immunology·Franziska BayerChristoph Reinhardt
Nov 16, 2018·Frontiers in Physiology·Elizabeth A KennedyMegan T Baldridge
Nov 17, 2020·Frontiers in Oncology·Yen-Cheng ChenJaw-Yuan Wang
Feb 16, 2021·Journal of Lipid Research·Maryam HonarbakhshLoredana Quadro
Feb 27, 2021·International Journal of Medical Microbiology : IJMM·Marijana BasicAndré Bleich
Aug 14, 2021·Translational Vision Science & Technology·Asadolah MovahedanDimitra Skondra
Sep 19, 2021·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M-C BirlingL Teboul

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