Maintaining HNF6 expression prevents AdHNF3beta-mediated decrease in hepatic levels of Glut-2 and glycogen

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Yongjun TanRobert H Costa

Abstract

The hepatocyte nuclear factor 3 (HNF-3) proteins are members of the Forkhead Box (Fox) family of transcription factors that play important roles in regulating expression of genes involved in cellular proliferation, differentiation, and metabolic homeostasis. In previous studies we increased liver expression of HNF-3beta by using either transgenic mice (transthyretin HNF-3beta) or recombinant adenovirus infection (AdHNF3beta), and observed diminished hepatic levels of glycogen, and glucose transporter 2 (Glut-2), as well as the HNF-6, HNF-3, HNF-1alpha, HNF-4alpha, and C/EBPalpha transcription factors. We conducted the present study to determine whether maintaining HNF-6 protein expression during AdHNF3beta infection prevents reduction of hepatic levels of glycogen and the earlier-mentioned genes. Here, we show that AdHNF3beta- and AdHNF6-infected mouse liver displayed increased hepatic levels of glycogen, Glut-2, HNF-3gamma, HNF-1alpha, and HNF-4alpha at 2 and 3 days postinfection (PI). Furthermore, restoration of hepatic glycogen levels after AdHNF3beta and AdHNF6 coinfection was associated with increased Glut-2 expression. AdHNF6 infection alone caused a 2-fold increase in hepatic Glut-2 levels, suggesting that HNF 6 stimulat...Continue Reading

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Jan 12, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Yongjun TanRobert H Costa

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Citations

May 25, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ai-Xuan L HoltermanRobert H Costa
Aug 2, 2005·Molecular and Cellular Biology·Nir E RubinsKlaus H Kaestner
May 31, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Minhua WangAi-Xuan Holterman
Dec 7, 2007·Genome Biology·Zihua HuJames F Collins
Nov 25, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Nicolas Plumb-RudewiezFrédéric P Lemaigre
Sep 7, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Minhua WangAi-Xuan L Holterman
Dec 4, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Robert H CostaXinhe Wang
May 30, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Douglas E HughesRobert H Costa
Jan 28, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Yuichi YoshidaRobert H Costa
Jun 25, 2005·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·Marty H PorterPeter M Thulé
Jan 20, 2018·American Journal of Physiology. Endocrinology and Metabolism·Peter A KroppMaureen Gannon
Oct 30, 2015·Diseases·Alessandra MarchettiMarco Tripodi

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