Maintaining Specimen Integrity for G6PD Screening by Cytofluorometric Assays

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Maria KahnGonzalo J Domingo

Abstract

Cytochemical staining remains an efficient way of identifying females who are heterozygous for the X chromosome-linked glucose-6-phosphate dehydrogenase (G6PD) gene. G6PD is highly polymorphic with certain alleles resulting in low intracellular G6PD activity in red blood cells. Low intracellular G6PD activity is associated with a risk of severe hemolysis when exposed to an oxidative stress such as fava beans, certain drugs and infections. Heterozygous females express the enzyme from both X-chromosome alleles resulting in two red blood cell populations each with G6PD enzyme characteristics representative of each allele; for example, normal and deficient. Cytochemical staining is the only way to determine the relative representation of each allele in red blood cells, a feature that is critical when assessing the risk for severe hemolysis when exposed to an oxidant such as the anti-malarial drug primaquine. This letter discusses red blood cell integrity with respect to the cytofluorometric assays for G6PD activity. An approach to making this test more robust is suggested. The approach makes this test more reliable and extends its use to a broader range of blood specimens.

References

Sep 1, 1989·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C J Van NoordenJ Aten
Jun 24, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Anna L Peters, Cornelis J F Van Noorden
Nov 26, 2009·Journal of Proteomics·Niels LionJean-Daniel Tissot

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Citations

Dec 29, 2016·PloS One·Jeanette Krogh PetersenBjarne Winther Kristensen
Sep 13, 2017·Malaria Journal·Benedikt LeyRic N Price
Dec 15, 2017·European Journal of Haematology·Michael KalnokyGonzalo J Domingo
Jan 9, 2021·Tropical Medicine & International Health : TM & IH·Alpeshkumar Bipinbhai KapadiaNeelam Varma
Mar 24, 2021·The Journal of Applied Laboratory Medicine·Victoria HigginsDavor Brinc

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Methods Mentioned

BETA
flow cytometry
Assay
Flow

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