Maintaining the ribosomal reading frame: the influence of the E site during translational regulation of release factor 2

Cell
Viter MárquezK H Nierhaus

Abstract

Maintenance of the translation reading frame is one of the most remarkable achievements of the ribosome while decoding the information of an mRNA. Loss of the reading frame through spontaneous frameshifting occurs with a frequency of one in 30,000 amino acid incorporations. However, at many recoding sites, the mechanism that controls reading frame maintenance is switched off. One such example is the programmed +1 frameshift site of the prfB gene encoding the termination factor RF2, in which slippage into the forward frame by one nucleotide can attain an efficiency of approximately 100%, namely, four orders of magnitude higher than normally observed. Here, using the RF2 frameshift window, we demonstrate that premature release of the E site tRNA from the ribosome is coupled with high-level frameshifting. Consistently, in a minimal system, the presence of the E site tRNA prevents the +1 frameshift event, illustrating the importance of the E site for reading-frame maintenance.

References

Oct 20, 1990·Journal of Molecular Biology·F Jørgensen, C G Kurland
Sep 5, 1988·Journal of Molecular Biology·J F Curran, M Yarus
Jan 1, 1987·Cold Spring Harbor Symposia on Quantitative Biology·R B WeissR F Gesteland
Jan 1, 1982·Bio Systems·N V BelitsinaA S Spirin
Aug 31, 1981·FEBS Letters·N V BelitsinaA S Spirin
Nov 1, 1994·Journal of Bacteriology·B LarsenJ F Atkins
Jun 1, 2000·Methods in Enzymology·F J Triana-AlonsoK H Nierhaus
Apr 3, 2001·Science·M M YusupovH F Noller
Mar 19, 2002·EMBO Reports·Pavel V BaranovJohn F Atkins
May 24, 2003·Cold Spring Harbor Symposia on Quantitative Biology·G Blaha, K H Nierhaus
May 27, 2003·Trends in Biochemical Sciences·James M OgleV Ramakrishnan
Aug 6, 2003·Angewandte Chemie·Daniel N Wilson, Knud H Nierhaus

❮ Previous
Next ❯

Citations

Jan 17, 2012·Protein & Cell·Xiang WangWeimin Gong
Mar 29, 2012·Journal of the American Chemical Society·Shuntaro TakahashiYoshio Okahata
Dec 19, 2008·Nature·Hani S Zaher, Rachel Green
Nov 17, 2010·Nature Reviews. Microbiology·Noah M ReynoldsMichael Ibba
Jan 28, 2009·Nature Structural & Molecular Biology·Sean R ConnellChristian M T Spahn
Sep 26, 2006·Nature Structural & Molecular Biology·Barbara S SchuwirthAntón Vila-Sanjurjo
Aug 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Viviana Di GiaccoKnud H Nierhaus
Mar 2, 2012·The Journal of Biological Chemistry·Alexandros D Petropoulos, Rachel Green
Oct 26, 2005·Nucleic Acids Research·Petr V SergievOlga A Dontsova
Jul 18, 2008·Nucleic Acids Research·Tatyana V BudkevichKnud H Nierhaus
Sep 29, 2009·Nucleic Acids Research·Pei-Yu LiaoKelvin H Lee
Mar 5, 2009·Microbiology and Molecular Biology Reviews : MMBR·John F Atkins, Glenn R Björk
Jun 15, 2005·Annual Review of Biochemistry·James M Ogle, V Ramakrishnan
Oct 10, 2008·BMC Genomics·Age TatsMaido Remm
Jul 31, 2007·RNA·Christina L Sanders, James F Curran
Oct 28, 2008·Biological Chemistry·Stefano MarziThomas Geissmann
Feb 11, 2014·Mathematical Biosciences·Ping Xie
Jul 4, 2015·International Journal of Molecular Sciences·Howard B GamperYa-Ming Hou
Jan 26, 2016·Nature Structural & Molecular Biology·Dejiu ZhangYan Qin
Dec 2, 2011·Computational Biology and Chemistry·Christian J Michel
Nov 18, 2008·Journal of Molecular Biology·S Joakim NäsvallGlenn R Björk
Nov 26, 2015·Molecular Cell·Angelica FergusonScott C Blanchard
Mar 2, 2016·Nature Structural & Molecular Biology·Michael R WassermanScott C Blanchard
Mar 20, 2015·Proceedings of the National Academy of Sciences of the United States of America·Joshua P RamsayClive W Ronson
Feb 18, 2010·Structure·John F FlanaganRobert J C Gilbert
Sep 24, 2016·Quarterly Reviews of Biophysics·Jin ChenJoseph D Puglisi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.