Maintenance of claudin-3 expression and the barrier functions of intercellular junctions in parotid acinar cells via the inhibition of Src signaling

Archives of Oral Biology
Megumi YokoyamaJunko Fujita-Yoshigaki

Abstract

Salivary acinar and duct cells show different expression patterns of claudins, which may reflect their different functions. To study the role of claudins in saliva secretion, we examined alterations in the expression patterns of cell adhesion molecules in parotid glands of γ-irradiated rats and analyzed the influence of those changes on intercellular barrier function using primary cultures of parotid acinar cells. Rats were γ-irradiated with doses of 5, 15 or 20Gy, and expression levels of cell adhesion molecules were examined by immunoblotting analysis. Acinar cells were isolated from parotid glands and were cultured in the absence or presence of the Src kinase inhibitor PP1. Changes in protein and mRNA expression patterns were determined by immunoblotting and by RT-PCR analyses, respectively. Intercellular barrier function was examined by measuring transepithelial electrical resistance and the paracellular flux of FITC-dextran. In irradiated parotid glands, the expression of claudin-4 was enhanced at 15Gy or higher, levels that induce the hyposecretion of saliva, although that increase was transient. At 30days after irradiation, expression levels of cell adhesion molecules were decreased. In primary cultures, the expression o...Continue Reading

Citations

Jun 13, 2019·Oncology Letters·Xinglong YeJing Kang
Apr 21, 2019·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Lian Bi, Peter Lwigale
Jan 14, 2021·American Journal of Physiology. Cell Physiology·Shaista AnwerKatalin Szászi
Nov 2, 2018·Archives of Oral Biology·Jomy J VargheseDanielle S W Benoit

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