Maintenance of DNA methylation level in SV40-infected human fibroblasts during their in vitro limited proliferative life span.

Experimental Cell Research
T MatsumuraR Holliday

Abstract

Methylation level as expressed by the molar ratio of 5-methylcytosine content to the combined content of cytosine and 5-methylcytosine was determined by HPLC and uv adsorption of cellular DNA extracted from SV40-infected and pretransformed MRC-5 human diploid fibroblasts (HDFs) during their limited in vitro life span. The level decreased slightly during early passages, and then was maintained within a certain range in the subsequent pretransformed stage of serial passages. When HDFs were treated with 5-aza-2'-deoxycytidine (5-aza-CdR) at an effective concentration shortly after the SV40 infection, the level decreased and then increased or was maintained again within a certain range in the subsequent pretransformed state. The proliferative life span potential of SV40-infected HDFs was not significantly decreased by the 5-aza-CdR treatment. These results are in contrast to the established observations for uninfected HDFs, that methylation level decreases during serial passages, and that, after treatment with 5-aza-CdR, the level, as well as the proliferative life span, is decreased in comparison to untreated populations. These results show that SV40-infected pretransformed HDFs are in an intermediate state between normal finite g...Continue Reading

References

May 1, 1979·Journal of Gerontology·T MatsumuraL Hayflick
Jan 1, 1987·Experimental Cell Research·D S FairweatherG P Margison
Feb 1, 1987·Cell Biology International Reports·S Honda, M Matsuo
Oct 9, 1987·Science·R Holliday
Mar 29, 1974·Nature·A Macieira-Coelho
Oct 1, 1984·Molecular and Cellular Biology·E FlatauP A Jones
Jun 3, 1983·Science·V L Wilson, P A Jones
Sep 2, 1983·Science·O M Pereira-Smith, J R Smith
Dec 1, 1961·Experimental Cell Research·L HAYFLICK, P S MOORHEAD

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Citations

Jul 1, 1991·Somatic Cell and Molecular Genetics·R E GilesW W Brockman
Jan 1, 1989·Experimental Gerontology·T MatsumuraR Holliday
Jun 28, 2002·Experimental Gerontology·Robin Holliday
Dec 10, 1999·Critical Reviews in Oncology/hematology·J P Issa
Nov 1, 1990·Experimental Cell Research·S Linder, H Marshall

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