Maintenance of Mest imprinted methylation in blastocyst-stage mouse embryos is less stable than other imprinted loci following superovulation or embryo culture

Environmental Epigenetics
Brenna A M VelkerMellissa R W Mann

Abstract

Assisted reproductive technologies are fertility treatments used by subfertile couples to conceive their biological child. Although generally considered safe, these pregnancies have been linked to genomic imprinting disorders, including Beckwith-Wiedemann and Silver-Russell Syndromes. Silver-Russell Syndrome is a growth disorder characterized by pre- and post-natal growth retardation. The Mest imprinted domain is one candidate region on chromosome 7 implicated in Silver-Russell Syndrome. We have previously shown that maintenance of imprinted methylation was disrupted by superovulation or embryo culture during pre-implantation mouse development. For superovulation, this disruption did not originate in oogenesis as a methylation acquisition defect. However, in comparison to other genes, Mest exhibits late methylation acquisition kinetics, possibly making Mest more vulnerable to perturbation by environmental insult. In this study, we present a comprehensive evaluation of the effects of superovulation and in vitro culture on genomic imprinting at the Mest gene. Superovulation resulted in disruption of imprinted methylation at the maternal Mest allele in blastocysts with an equal frequency of embryos having methylation errors follow...Continue Reading

References

Apr 1, 1995·Human Molecular Genetics·D KotzotM Hergersberg
May 1, 1997·Human Molecular Genetics·S KobayashiF Ishino
Sep 25, 1997·Human Molecular Genetics·L LefebvreM A Surani
Nov 20, 2001·The Journal of Biological Chemistry·Yayoi Obata, Tomohiro Kono
Mar 8, 2002·The New England Journal of Medicine·Laura A SchieveLynne S Wilcox
May 23, 2002·American Journal of Human Genetics·Gerald F CoxBernhard Horsthemke
Nov 20, 2002·American Journal of Human Genetics·Michael R DeBaunAndrew P Feinberg
Jan 15, 2003·Journal of Medical Genetics·E R MaherM M Hawkins
Mar 5, 2004·Human Molecular Genetics·Diana LuciferoJacquetta M Trasler
Jul 9, 2004·Development·Mellissa R W MannMarisa S Bartolomei
Mar 22, 2005·The Journal of Biological Chemistry·Takuya ImamuraAndràs Pàldi
Apr 14, 2006·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hitoshi HiuraTomohiro Kono
Aug 23, 2006·Human Reproduction·A SatoT Arima
Oct 7, 2009·Human Molecular Genetics·Brenna A Market-VelkerMellissa R W Mann
Jul 10, 2010·The Journal of Molecular Diagnostics : JMD·Jochen K LennerzBarbara A Zehnbauer
Aug 31, 2010·American Journal of Medical Genetics. Part C, Seminars in Medical Genetics·Thomas Eggermann
Sep 23, 2011·Epigenetics : Official Journal of the DNA Methylation Society·Nady El HajjThomas Haaf
Aug 8, 2013·Pediatrics International : Official Journal of the Japan Pediatric Society·Hatsune ChibaTakahiro Arima

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Citations

Dec 28, 2017·Molecular Reproduction and Development·Kira L Marshall, Rocio Melissa Rivera
Feb 19, 2020·Annual Review of Animal Biosciences·Peter J Hansen
Aug 29, 2019·International Journal of Molecular Sciences·Marina La RovereLiborio Stuppia

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