PMID: 2098703Oct 1, 1990Paper

Maintenance of proton motive force by Streptococcus mutans and Streptococcus sobrinus during growth in continuous culture

Oral Microbiology and Immunology
I R Hamilton

Abstract

The components of the transmembrane electrochemical proton gradient, or proton motive force (PMF, delta p), were determined in cells of Streptococcus mutans Ingbritt and Streptococcus sobrinus ATCC 27352 growing in continuous culture under conditions of changing glucose concentration, growth rate and growth pH. The pH gradient (delta pH) and membrane electrical potential (delta psi) were assayed with the weak acid, salicyclic acid, and the lipophilic cation, methyltriphenylphosphonium iodide, respectively. S. mutans Ingbritt growing in continuous culture (pH 7.0, dilution rate (D) = 0.1 h-1) at 8 glucose concentrations ranging from 2.8 to 288 mM maintained a relatively constant delta p of 58.3 mV (SD +/- 5.8) in spite of a transition from glucose to nitrogen-limited growth and significant changes in cell physiology. Changes included a decreasing yield constant, increasing glucose uptake rates in the chemostat, repression of Ellglc of the PEP phosphotransferase sugar transport system and decreasing glycolytic capacity of the cells as the medium glucose concentration increased. Changes in the dilution or growth rate of S. mutans Ingbritt from 0.1 to 1.0 h-1 and S. sobrinus from 0.1 to 0.8 h-1, when growing at pH 7.0 with limited ...Continue Reading

References

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Citations

Apr 1, 1991·Oral Microbiology and Immunology·I R Hamilton, N D Buckley
Mar 6, 1998·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·G H Bowden, I R Hamilton
Feb 10, 2004·Applied and Environmental Microbiology·Elizabeth M Fozo, Robert G Quivey
Mar 10, 2010·Future Microbiology·Robert Matsui, Dennis Cvitkovitch
Apr 1, 1997·Advances in Dental Research·J Carlsson
Nov 10, 1998·Oral Microbiology and Immunology·I R Hamilton, G Svensäter
May 11, 2004·Microbiology·Alice C L LenNicholas A Jacques
Jun 1, 1993·Archives of Oral Biology·C H Sissons, E M Hancock

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