PMID: 7007820Jan 1, 1980Paper

Maintenance of some ColE1-type plasmids in chemostat culture

Molecular & General Genetics : MGG
I M JonesD C Ellwood

Abstract

When cells carrying the plasmids RP1, pDS4101 (a ColK derivative) or pDS1109 (a ColE1 derivative) were maintained in chemostat culture in the absence of antibiotic selection, plasmid-free segregants were not detected after 120 generations of nutrient-limited growth. By contrast, plasmid-free segregants of pMB9- and pBR322-containing cells arose after approximately 30 generations, irrespective of the host genetic background. However, even though pDS1109 was maintained its copy-number fell five-fold during 80 generations of limited growth. It is suggested that loss of pBR322 occurs following a similar copy-number decrease which results in defective segregation of the plasmid to daughter host cells. This defective segregation was not complemented in trans by either RP1 or pDS4101.

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Citations

Apr 1, 1999·Biotechnology and Bioengineering·R R SharpM S Shields
Apr 1, 1999·Biotechnology and Bioengineering·R R SharpW G Jones
Jan 1, 1982·Molecular & General Genetics : MGG·I M JonesS D Ehrlich
Jan 1, 1983·Molecular & General Genetics : MGG·R M WalmsleyS G Oliver
Mar 1, 1984·Microbial Ecology·P C Gowland, J H Slater
Feb 1, 1991·Applied Biochemistry and Biotechnology·M KobayashiH Yukawa
Jun 1, 1990·Journal of Biotechnology·P K Kumar, K Schügerl
Nov 1, 1983·Gene·P R Rosteck, C L Hershberger
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Jan 1, 1984·Biotechnology & Genetic Engineering Reviews·R H Doi
Jan 1, 1987·Annals of the New York Academy of Sciences·F M IníestaJ N Barbotin
Jan 1, 1986·Annals of the New York Academy of Sciences·D D Ryu, R Siegel
Dec 29, 2004·FEMS Microbiology Letters·Stephanie AbromaitisFrance Daigle
Jul 6, 2016·Frontiers in Microbiology·Margaret M McDanielVitaly V Ganusov
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