Maintenance requirements for bacteria growing on C1-compounds

Biotechnology and Bioengineering
J S RokemR I Mateles

Abstract

The maintenance coefficient, ms (mmol substrate/g cell dry wt hr), of two distinct groups of C1-utilizing bacteria has been determined by growing the organisms in an aerobic continuous culture limited by different C1 growth substrates. For growth on methanol, ms = 2.5 +/- 0.3 for Pseudomonas C; 3.9 +/- 0.7 for Ps. methylotropha (these bacteria utilize methanol via the ribulose monophosphate pathway of formaldehyde fixation); 1.5 +/- 0.2 for Pseudomonas 1, and 2.3 +/- 0.4 for Pseudomonas 135 (the latter bacteria utilize C1-compounds via the serine pathway). For growth on formaldehyde, ms = 1.5 +/- 0.3 for Pseudomonas 1 and 2.7 +/- 0.7 for Pseudomonas 135, whereas on formate the values for ms are 1.0 +/- 0.2 and 4.4 +/- 1.3; respectively. Although the maintenance coefficients did not differ systematically between the two groups of bacteria, the maintenance requirements per generation of the serine pathway bacteria were considerably higher (8.7 vs. 3.9) owing to their slower growth rate. The maximum molar yield values, YMmax (g cell dry wt/mol substrate utilized), corrected for the maintenance energy of bacteria which utilize C1-compounds via the ribulose monophosphate pathway averaged 19.1 when grown on methanol, while the values...Continue Reading

References

Dec 1, 1976·Biotechnology and Bioengineering·I GoldbergR I Mateles
Mar 1, 1976·Canadian Journal of Microbiology·E Bellion, J C Spain
Oct 12, 1965·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·S J Pirt
Dec 1, 1972·Journal of General Microbiology·S Nagai, S Aiba
May 1, 1963·The Biochemical Journal·P J Large, J R Quayle

Related Concepts

Adenosine Triphosphate, Chromium Ammonium Salt
Sodium Methoxide
Vitreous Carbon
Formol
Pseudomonas
Ribulosephosphates

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