PMID: 2125202Aug 1, 1990Paper

Maize genotypes classified as null at the glu locus have beta-glucosidase activity and immunoreactive protein

Biochemical Genetics
A Esen, C Cokmus

Abstract

Maize beta-glucosidase (beta-D-glucoside glucohydrolase; EC 3.2.1.21) was extracted from coleoptiles of 15 maize genotypes (3 normals, 10 nulls, and 2 hybrids) in two fractions, the soluble and the insoluble. The enzyme activity was measured spectrophotometrically in the soluble fraction and also studied on zymograms after native gel electrophoresis and isoelectric focusing. The enzyme was purified from a normal genotype by anion-exchange chromatography and preparative electrophoresis. Antisera were raised in four rabbits, and the soluble and the insoluble extracts of each genotype were analyzed for a cross-reacting material by ELISA and immunoblotting. The results showed that extracts from both the normal and the null genotypes had beta-glucosidase activity, and the activity measured spectrophotometrically was 2- to 10-fold higher in normals than in nulls. Zymograms of the null genotypes were devoid of distinct bands that were present in those of normals and hybrids from crosses between normals and nulls. Zymograms of both the normal and the null genotypes had a diffuse, smeared zone of activity at the cathodic end of native gels. A cross-reacting antigen was present in extracts of both genotypes when assayed by ELISA and a 60...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Dec 28, 1964·Annals of the New York Academy of Sciences·L ORNSTEIN
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS

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Citations

Dec 3, 1999·European Journal of Biochemistry·V RotreklB Brzobohatý
Dec 1, 1994·Plant Molecular Biology·B BrzobohatýK Palme
Apr 1, 1999·Biotechnology and Bioengineering·M Cicek, A Esen
Feb 21, 2006·Journal of Experimental Botany·Nagavalli S KiranBretislav Brzobohaty
May 13, 2008·Phytochemistry·Anne Vinther MorantSøren Bak

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