PMID: 11607198Jul 15, 1991Paper

Maize oleosin is correctly targeted to seed oil bodies in Brassica napus transformed with the maize oleosin gene

Proceedings of the National Academy of Sciences of the United States of America
W S LeeA H Huang

Abstract

Oleosins are small hydrophobic abundant proteins localized in the oil bodies of plant seeds. An oleosin gene from the monocotyledonous maize (Zea mays L.) was transferred into the dicotyledonous Brassica napus L. using Agrobacterium-mediated transformation. The maize oleosin gene was placed under the control of either its own promoter/terminator or the promoter/terminator of a Brassica seed storage protein (napin) gene. Southern blot analyses of individual transformed plants suggested that the oleosin gene from either construct was incorporated into the Brassica chromosomes without appreciable structural alterations. The amount of construct incorporated was from 1 to >10 copies per haploid genome, depending on the individual transformant. Maize oleosin mRNA and protein were detected only in the transformants containing the napin gene promoter/terminator constructs; these transformants were studied further. Northern blot analyses of RNA isolated from different tissues and seeds of different developmental stages indicated that the maize oleosin mRNA was present only in the maturing seed. Approximately 1% of the total protein in mature seed was represented by maize oleosin. Subcellular fractionation of the mature seed revealed tha...Continue Reading

Citations

Oct 1, 1992·Plant Molecular Biology·S Y Bednarek, N V Raikhel
Jan 1, 1994·Progress in Lipid Research·D J Murphy
Sep 22, 2000·Biochimica Et Biophysica Acta·D ZweytickG Daum
Jul 28, 2001·Physiologia Plantarum·Gitte I. FrandsenJason T. C. Tzen
Apr 1, 1992·The Journal of Cell Biology·J T Tzen, A H Huang
Apr 1, 1992·Plant Molecular Biology·G J van RooijenM M Moloney
Aug 28, 2012·Journal of Biotechnology·Somrutai WinichayakulNick Roberts
Jun 6, 2009·Plant Biotechnology Journal·Loreta Gudynaite-SavitchBrian L A Miki
Aug 12, 2014·PloS One·Yuan FangBrent D Mishler
Feb 7, 1997·The Journal of Biological Chemistry·J T TingA H Huang
Feb 10, 2020·Molecular Biotechnology·Padikara Kutty Satheeshkumar
Aug 1, 2006·The Plant Cell·Rodrigo M P SilotoMaurice M Moloney
Feb 9, 2013·Plant Physiology·Nan-Lan HuangAnthony H C Huang

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