Maize Urb2 protein is required for kernel development and vegetative growth by affecting pre-ribosomal RNA processing

The New Phytologist
Hongqiu WangJihua Tang

Abstract

Ribosome biogenesis is a fundamental process in eukaryotic cells. Although Urb2 protein has been implicated in ribosome biogenesis in yeast, the Urb2 domain is loosely conserved between plants and yeast, and the function of Urb2 protein in plants remains unknown. Here, we isolated a maize mutant, designated as urb2, with defects in kernel development and vegetative growth. Positional cloning and transgenic analysis revealed that urb2 encodes an Urb2 domain-containing protein. Compared with the wild-type (WT), the urb2 mutant showed decreased ratios of 60S/40S and 80S/40S and increased ratios of polyribosomes. The pre-rRNA intermediates of 35/33S rRNA, P-A3 and 18S-A3 were significantly accumulated in the urb2 mutant. Transcriptome profiling of the urb2 mutant indicated that ZmUrb2 affects the expression of a number of ribosome-related genes. We further demonstrated that natural variations in ZmUrb2 are significantly associated with maize kernel length. The overall results indicate that, by affecting pre-rRNA processing, the Urb2 protein is required for ribosome biogenesis in maize.

References

Oct 10, 1981·Nucleic Acids Research·L H BowmanD Schlessinger
Mar 6, 2003·Neuroscience Letters·Christian RamakersAntoon F M Moorman
May 24, 2003·Cold Spring Harbor Symposia on Quantitative Biology·J R WarnerJ H Sohn
Jul 3, 2003·Cell·Wen Tao PengTimothy R Hughes
Jul 10, 2004·Cell·Sanie MnaimnehTimothy R Hughes
Aug 7, 2004·Bioinformatics·J C BarrettM J Daly
Jun 28, 2005·Trends in Biotechnology·James S McLaren
Dec 28, 2006·Cell·John F DoebleyBruce D Smith
May 22, 2007·Biopolymers·Katrin Karbstein
Jun 26, 2007·Bioinformatics·Peter J BradburyEdward S Buckler
Apr 15, 2008·Cellular and Molecular Life Sciences : CMLS·A K HenrasY Henry
Jul 10, 2009·Methods in Molecular Biology·Angelika MustrophJulia Bailey-Serres
Jan 26, 2010·Trends in Biochemical Sciences·Denis L J Lafontaine
Mar 27, 2010·Nucleic Acids Research·Monika Zakrzewska-PlaczekJoanna Kufel
Jun 21, 2011·The Plant Journal : for Cell and Molecular Biology·Heike LangeDominique Gagliardi
Dec 27, 2011·The Plant Cell·Amanda J WatersNathan M Springer
Feb 22, 2012·Biochimie·Sahra-Taylor Mullineux, Denis L J Lafontaine
Nov 6, 2013·Genetics·John L Woolford, Susan J Baserga
Oct 17, 2014·The Plant Journal : for Cell and Molecular Biology·Benjamin L WeisEnrico Schleiff
Oct 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Runlai HangXiaofeng Cao
Nov 30, 2014·BMC Plant Biology·Hui-Li XingQi-Jun Chen
Dec 23, 2014·Nature Genetics·Weiliang ZuoMingliang Xu
Mar 10, 2015·Nature Methods·Daehwan KimSteven L Salzberg
Oct 16, 2015·Trends in Plant Science·Benjamin L WeisEnrico Schleiff
Oct 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Pan ZhuDanmeng Zhu
May 4, 2017·Proceedings of the National Academy of Sciences of the United States of America·Nelson GarciaJoachim Messing

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.