Maize WI5 encodes an endo-1,4-β-xylanase required for secondary cell wall synthesis and water transport in xylem.

Journal of Integrative Plant Biology
Xiaojiao HuHaiming Zhao

Abstract

Water transport from roots to leaves through xylem is important for plant growth and development. Defects in water transport can cause drought stress, even when there is adequate water in the soil. Here, we identified the maize (Zea mays) wilty5 (wi5) mutant, which exhibits marked dwarfing and leaf wilting throughout most of its life cycle under normal growth conditions. wilty5 seedlings exhibited lower xylem conductivity and wilted more rapidly under drought, NaCl, and high temperature treatments than wild-type plants. Map-based cloning revealed that WI5 encodes an active endo-1,4-β-xylanase from glycosyl dehydration family 10, which mainly functions in degrading and reorganizing cell wall xylan. Reverse-transcription polymerase chain reaction and β-glucuronidase assays revealed that WI5 is highly expressed in stems, especially in internodes undergoing secondary wall assembly. RNA sequencing suggested that WI5 plays a unique role in internode growth. Immunohistochemistry and electron microscopy confirmed that wi5 is defective in xylan deposition and secondary cell wall thickening. Lignin deposition and xylan content were markedly reduced in wi5 compared to the wild-type plants. Our results suggest that WI5 functions in xylem c...Continue Reading

References

Apr 26, 2000·Genome Research·A A Salamov, V V Solovyev
Jun 8, 2001·The Plant Journal : for Cell and Molecular Biology·M P CaspersV Cameron-Mills
Jul 7, 2005·The Plant Journal : for Cell and Molecular Biology·Zhizhong ChenZhizhong Gong
Oct 3, 2006·Current Opinion in Plant Biology·Olivier LerouxelKenneth Keegstra
Apr 25, 2008·Current Opinion in Plant Biology·John Vogel
Oct 8, 2008·Nucleic Acids Research·Brandi L CantarelBernard Henrissat
Nov 28, 2008·The Plant Journal : for Cell and Molecular Biology·Thorsten HamannChristopher Somerville
Feb 28, 2009·Journal of Experimental Botany·Mei ZhangPing Leng
Feb 16, 2010·Plant Physiology·Georg J Seifert, Claudia Blaukopf
Mar 13, 2010·Journal of Visualized Experiments : JoVE·Cliff E FosterMarkus Pauly
Mar 17, 2010·Critical Reviews in Biotechnology·Annick PolletChristophe M Courtin
Apr 23, 2010·The Plant Journal : for Cell and Molecular Biology·Jianhua ZhuJian-Kang Zhu
Dec 6, 2011·Biotechnology Advances·Veeresh Juturu, Jin Chuan Wu
Jan 10, 2012·Annual Review of Plant Biology·Sebastian WolfHerman Höfte
Jan 1, 2014·Proceedings of the National Academy of Sciences of the United States of America·Chien Van HaLam-Son Phan Tran
Mar 11, 2015·Journal of Integrative Plant Biology·Magdalena GlassShawn D Mansfield
Jun 27, 2015·Nature Communications·Artur RogowskiDavid N Bolam
Jul 15, 2015·Glycobiology·Abhishek A Kognole, Christina M Payne
Jul 15, 2015·Journal of Integrative Plant Biology·Chiming GuoLiang Chen
Sep 24, 2015·Proceedings of the National Academy of Sciences of the United States of America·Qun WanAndrey Kovalevsky
Oct 28, 2015·Journal of Integrative Plant Biology·Shuang-Shuang ZhangJian-Xiang Liu
Sep 14, 2016·Scientific Reports·Vasilios M E AndriotisAlison M Smith
Mar 16, 2017·Journal of Integrative Plant Biology·Martin D VenturasUwe G Hacke
Sep 30, 2017·The Plant Journal : for Cell and Molecular Biology·Wen-Qiang LiKun-Ming Chen
Dec 22, 2017·The Plant Journal : for Cell and Molecular Biology·Laura BaceteAntonio Molina
Feb 3, 2018·Journal of Integrative Plant Biology·Jun-Bo HeAi-Min Wu

❮ Previous
Next ❯

Datasets Mentioned

BETA
AC208082

Methods Mentioned

BETA
PCR
Transgenic
PCA

Software Mentioned

DESeq2
DNAMAN
SNP2CAPS
Primer
PROSITE
Basic Local Alignment Search Tool
FGENESH
SSR Hunter
MEGA

Related Concepts