PMID: 7538518May 1, 1995Paper

Major differences in specificity among naturally occurring human IgG-subclass anti-IgE autoantibodies

The Journal of Allergy and Clinical Immunology
C G Magnusson

Abstract

The specificity of naturally occurring IgG-subclass anti-IgE autoantibodies (a-IgE Ab) was studied by ELISA inhibition assay with a highly purified IgE-DES myeloma protein as a solid-phase antigen. Because the IgG isotypes differ in effector functions, detailed specificity studies of IgG subclass anti-IgE antibodies might clarify their putative role. Selected sera containing a high concentration of a single IgG a-IgE Ab subclass were allowed to react with purified immunoglobulins of all five classes including five different IgE myeloma proteins and papain-derived Fab epsilon and Fc epsilon fragments of IgE-DES. The inhibition results indicated that IgG1, IgG2, and IgG3 a-IgE Ab reacted in a low-affinity reaction with IgE myeloma protein-restricted determinants because only IgE-DES was inhibitory. Moreover, the reacting epitopes were heat resistant (2 hours, 56 degrees C) and localized in the Fab epsilon-DES fragment. In sharp contrast, IgG4 a-IgE Ab reacted with high affinity to all five IgE myeloma proteins involving heat-susceptible epitopes confined to the Fc epsilon fragment. It seems that a majority of IgG a-IgE Ab have a specificity for nonisotypic myeloma-restricted determinants whereas IgG4 a-IgE Ab are mainly isotype s...Continue Reading

Citations

Aug 1, 1995·Molecular Immunology·C WingrenM Ohlin
Jul 14, 1999·Molecular Immunology·J E BjörklundC G Magnusson
Mar 3, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·G MaroneG Condorelli
Feb 7, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M N SatoA J S Duarte
May 16, 1998·Autoimmunity·H M Cheng
May 3, 2000·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M KronqvistM van Hage-Hamsten

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