Major histocompatibility class II transactivator (CIITA) mediates repression of collagen (COL1A2) transcription by interferon gamma (IFN-gamma)

The Journal of Biological Chemistry
Yong XuBarbara D Smith

Abstract

Interferon gamma (IFN-gamma) plays an important role during inflammation by repressing collagen and activating major histocompatibility class II (MHC-II) expression. Activation of MHC-II by IFN-gamma requires regulatory factor for X-box 5 (RFX5) complex as well as class II transactivator (CIITA). We have shown that the RFX family binds to the COL1A2 transcription start site, and the RFX5 complex represses COL1A2 gene expression during IFN-gamma response. In this report, we demonstrate that CIITA is a key mediator of COL1A2 repression by IFN-gamma. IFN-gamma up-regulates the expression of CIITA in a time-dependent manner in lung fibroblasts and promotes CIITA protein occupancy on COL1A2 transcription start site in vivo as judged by chromatin immunoprecipitation (ChIP) assays. There are coordinate decreases in the occupancy of RNA polymerase II on the collagen transcription start site with increasing CIITA occupancy during IFN-gamma treatment. In addition, we are able to specifically knockdown the IFN-gamma-stimulated expression of CIITA utilizing short hairpin interference RNA (shRNA) against CIITA. This leads to the alleviation of COL1A2 repression and MHC-II activation by IFN-gamma. RFX5 recruits CIITA to the collagen site as ...Continue Reading

References

May 31, 1991·Biochemical and Biophysical Research Communications·G Karsenty, B de Crombrugghe
Jul 29, 1988·Science·S N MaityB de Crombrugghe
Sep 1, 1984·The Journal of Clinical Investigation·S A JimenezJ Rosenbloom
Aug 30, 1984·Biochemical and Biophysical Research Communications·J RosenbloomS A Jimenez
Jan 6, 1995·The Journal of Biological Chemistry·F CoustryB de Crombrugghe
Feb 24, 1995·The Journal of Biological Chemistry·G H MacDonaldJ P Ting
Jan 1, 1996·Annual Review of Immunology·B MachW Reith
Feb 1, 1997·Current Opinion in Immunology·J M Boss
Jun 15, 1997·Nucleic Acids Research·J D FontesB M Peterlin
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·S K MahantaJ L Strominger
Jul 18, 1997·Journal of Molecular Biology·J D FontesB M Peterlin
Jan 14, 1999·The Journal of Investigative Dermatology·S J ChenJ Varga
Dec 14, 1999·The Journal of Biological Chemistry·P K SenguptaB D Smith
Jul 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·D J TaxmanJ P Ting
Aug 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·T J SiskC H Chang
Feb 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·T S Gourley, C H Chang

❮ Previous
Next ❯

Citations

Nov 4, 2011·The Journal of Biological Chemistry·Larry L LuchsingerMatthew D Layne
Mar 25, 2005·The Journal of Biological Chemistry·Pritam SenguptaBarbara D Smith
Aug 14, 2010·American Journal of Respiratory Cell and Molecular Biology·Yong XuBarbara D Smith
Apr 26, 2008·PLoS Genetics·Michal KrawczykWalter Reith
Apr 18, 2008·Current Cardiology Reports·Mark P S Dunphy, H William Strauss
Mar 11, 2015·Nucleic Acids Research·Christopher D ScharerJeremy M Boss
Feb 8, 2005·Biochemical and Biophysical Research Communications·Sae-Bom JeonEun-Hye Joe
Oct 13, 2011·International Journal of Cancer. Journal International Du Cancer·Priya LondheJudith Davie
Aug 19, 2007·Circulation Research·Israel Gotsman, Andrew H Lichtman
Mar 4, 2006·Circulation Research·Marie-Luce Bochaton-Piallat, Giulio Gabbiani
Dec 6, 2006·Circulation·Euan A AshleyThomas Quertermous
Oct 18, 2006·Journal of Korean Medical Science·Tae Woon KimKyeong Cheon Jung
May 8, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Emily ChiuDrew E Cressman
Jun 21, 2005·The Journal of Biological Chemistry·Audrey H WangXiang-Jiao Yang
May 18, 2013·European Journal of Immunology·Mingming FangYong Xu
Sep 20, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hao H HoLionel B Ivashkiv
Jan 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christina S K YeeCheong-Hee Chang
Jan 28, 2021·International Journal of Molecular Sciences·Jorge Alfonso León Machado, Viktor Steimle
Jul 4, 2006·Matrix Biology : Journal of the International Society for Matrix Biology·Francesco RamirezGeorge Bou-Gharios
Dec 5, 2006·Matrix Biology : Journal of the International Society for Matrix Biology·Shizuko Tanaka, Francesco Ramirez
Nov 6, 2008·Clinics in Liver Disease·R UrtasunN Nieto

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.