Major histocompatibility complex class I allele-specific peptide libraries: identification of peptides that mimic an H-Y T cell epitope

European Journal of Immunology
M A GavinM J Bevan

Abstract

We describe a novel method for screening large libraries of random peptides for T cell antigens. Two libraries were constructed, containing fixed amino acids representing the major histocompatibility complex (MHC) class I anchor residues for H-2Kb-restricted octamers and H-2Db-restricted nonamers. Peptides from the Kb-restricted library (KbL: SXIXFXXL) and the Db-restricted library (DbL: XXXXNXXXIM) specifically stabilize empty Kb and Db molecules, respectively. The libraries contain peptides that mimic several H-2b-restricted cytotoxic T lymphocyte epitopes, and 21 mimotopes for a Db-restricted H-Y epitope were isolated. A degenerate synthetic peptide of limited complexity containing the identified H-Y sequence motif was found to be similar to the natural H-Y epitope by reverse-phase high performance liquid chromatography analysis. This peptide is also capable of immunizing female mice against male splenocytes. Several applications for MHC-restricted peptide libraries are discussed.

References

Nov 1, 1975·The Journal of Experimental Medicine·R D GordonL E Samelson
Aug 10, 1992·Journal of Immunological Methods·J A LenstraK K Stanley
Oct 1, 1992·The Journal of Experimental Medicine·J HammerF Sinigaglia
Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A G Grandea, M J Bevan
Nov 1, 1991·European Journal of Immunology·O RötzschkeH G Rammensee
Nov 1, 1991·The Journal of Experimental Medicine·O RötzschkeH G Rammensee
Aug 2, 1990·Nature·H G LjunggrenK Kärre
Mar 1, 1989·The Journal of Experimental Medicine·F R Carbone, M J Bevan
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A McLarenP S Burgoyne
Dec 1, 1985·The Journal of Experimental Medicine·H G Ljunggren, K Kärre
Apr 1, 1993·Current Opinion in Immunology·D P Lane, C W Stephen
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R A HendersonV H Engelhard
Jan 14, 1994·Cell·K A HogquistF R Carbone
Aug 1, 1993·The Biochemical Journal·I Saggio, R Laufer

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Citations

Oct 1, 1995·Current Opinion in Biotechnology·B Seed
Dec 1, 1996·Vaccine·P J Kuebler, D F Nixon
Feb 1, 1996·Current Opinion in Immunology·P Walden
Apr 12, 2003·Microbes and Infection·Jianzhu ChenDavid M Kranz
Dec 1, 1996·Nature Genetics·A GreenfieldP Koopman
May 10, 2000·Microbiology and Immunology·K KakugawaH Yamagishi
Dec 1, 1996·Immunological Reviews·J A Frelinger, M McMillan
Jun 8, 1999·Annual Review of Immunology·J W Yewdell, J R Bennink
Apr 20, 2004·PLoS Biology·Frances CrawfordJohn W Kappler
Sep 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·H S HiemstraJ W Drijfhout
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·R BrockP Walden
Jan 1, 1996·Trends in Biochemical Sciences·K Udaka
Jun 29, 2018·American Journal of Respiratory Cell and Molecular Biology·Shaikh M AtifClaudia V Jakubzick
Mar 20, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·L M MendozaN Shastri
Sep 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M MillrainP J Dyson
Jul 8, 2020·Nature Methods·Alok V Joglekar, Guideng Li
Mar 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·H E StefanskiK A Hogquist
Jul 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Denise M McKinneyMark J Newman
Aug 5, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·E BoenJ Bill
May 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rajwardhan YadavSebastian Joyce
Jun 3, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shaikh M AtifClaudia V Jakubzick

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